Thyroid hormone inhibits growth of hepatoma cells through induction of miR-214

Scientific Reports
Po-Shuan HuangKwang-Huei Lin

Abstract

Thyroid hormone (TH) plays a role in regulating the metabolic rate, heart functions, muscle control and maintenance of bones. 3,3'5-tri-iodo-L-thyronine (T3) displays high affinity to nuclear thyroid hormone receptors (TRs), which mediate most TH actions. Recent studies have shown hypothyroidism in patients with an increased risk of hepatocellular carcinoma (HCC). MicroRNAs (miRNAs), a class of non-protein-coding RNA, are suggested to control tumor growth by interacting with target genes. However, the clinical significance of T3/TR-regulated miRNAs in tumors has yet to be established. In the current study, miRNA expression profile screening was performed using SYBR Green-Based qRT-PCR array in TR-overexpressing HepG2 cells. miR-214-3p, which is expressed at low levels in HCC, was stimulated upon T3 application. The 3'UTR luciferase reporter assay confirmed that the proto-oncogene serine/threonine-protein kinase, PIM-1, is a miR-214-3p target. PIM-1 was decreased upon treatment with miR-214-3p or T3 stimulation. PIM-1 was highly expressed in HCC, and the effect of PIM-1 on cell proliferation might be mediated by the inhibition of p21. Furthermore, the T3-induced suppression of cell proliferation was partially rescued upon miR-21...Continue Reading

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Citations

Aug 24, 2018·Biomedicines·Shaker A MousaPaul J Davis
Jul 12, 2018·World Journal of Gastroenterology : WJG·Francesco VasuriDario de Biase
Mar 10, 2019·Journal of Biomedical Science·Hsiang-Cheng ChiKwang-Huei Lin
Oct 24, 2019·International Journal of Molecular Sciences·Po-Shuan HuangKwang-Huei Lin
Jan 27, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jianguo WuLi Wang
Jan 31, 2021·Molecular and Cellular Endocrinology·Ana Aranda
Oct 21, 2020·Biomedical Journal·Po-Shuan HuangKwang-Huei Lin

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Methods Mentioned

BETA
immunoprecipitation
ChIP
transfection
xenograft
PCR

Software Mentioned

TargetScan

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