Thyroid hormone receptor interacting protein 3 (trip3) is a novel coactivator of hepatocyte nuclear factor-4alpha

Diabetes
H IwahashiY Matsuzawa

Abstract

Mutations of the hepatocyte nuclear factor-4alpha (HNF-4alpha) gene are associated with a subtype of maturity-onset diabetes of the young (MODY1) that is characterized by impaired insulin secretion in response to a glucose load. HNF-4alpha, which is a transcription factor expressed in pancreatic beta-cells, plays an important role in regulating the expression of genes involved in glucose metabolism. Thus, cofactors that interact with HNF-4alpha and modify its transcriptional activity might also play an important role in regulating the metabolic pathways in pancreatic beta-cells, and the genes of such cofactors are plausible candidate genes for MODY. In the present study, we showed, using a yeast two-hybrid screening assay, that thyroid hormone receptor interacting protein 3 (Trip3) interacted with HNF-4alpha, and their interaction was confirmed by the glutathione S-transferase pull-down assay. Human Trip3 cDNA contained an open reading frame for a protein of 155 amino acids, and the gene was expressed in both pancreatic islets and MIN6 cells. Cotransfection experiments indicated that Trip3 could enhance (two- to threefold) the transcription activity of HNF-4alpha in COS-7 cells and MIN6 cells. These results suggest that Trip3 i...Continue Reading

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Citations

Jul 18, 2018·International Journal of Endocrinology·Chaoran ChenShu Fang Xia
Jul 28, 2007·Protein Science : a Publication of the Protein Society·Fahu HeShigeyuki Yokoyama
Apr 20, 2017·Diabetes Care·Danièle Dubois-LaforgueUNKNOWN Monogenic Diabetes Study Group of the Société Francophone du Diabète
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Philippe Cloutier, Benoit Coulombe
Mar 24, 2017·Brain : a Journal of Neurology·Anna-Kaisa AnttonenAnna-Elina Lehesjoki
Jul 29, 2021·Progress in Neuro-psychopharmacology & Biological Psychiatry·Florian KrullOle A Andreassen

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