Thyroid hormone receptor isoform content in cultured type 1 and type 2 astrocytes

Endocrinology
D J CarlsonJ H Oppenheimer

Abstract

Immunohistochemical studies previously reported from this laboratory showed that astrocytes in adult rat brain appear devoid of all thyroid hormone receptor (TR) isoforms. These findings, however, contrast with reports of measurable nuclear T3 binding in astrocytes in cell culture. To address this discrepancy, TR protein and messenger RNA (mRNA) content of type 1 and type 2 astrocytes in culture were assayed. Type 1 cells represent astrocytes present in brain in vivo. Type 2 astrocytes differentiate in culture from bipotential progenitor O-2A cells in the presence of serum. Under serum-free conditions, these progenitor cells differentiate into oligodendroglia. Total nuclear T3 binding capacity in both type 1 and type 2 astrocytes was approximately 3000 sites/cell. Northern blots showed the presence of mRNA for TRbeta1, TRalpha1, and TRalpha2 in type 2 cells but failed to reveal the presence of these mRNAs in type 1 astrocytes. Moreover, Northern blots also failed to reveal TRbeta2 mRNA in both type 1 and type 2 astrocytes. These findings, therefore, raised a question as to which receptor isoform was responsible for the nuclear binding capacity observed in type 1 astrocytes. As anticipated, immunocytochemical analysis demonstrat...Continue Reading

Citations

Aug 31, 2011·Journal of Thyroid Research·Petra MohácsikBalázs Gereben
Dec 28, 1999·BioFactors·J Bernal
Jul 12, 2008·Surgical Neurology·Shiuh-Lin HwangKung-Shing Lee
Nov 23, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Osama M AhmedR G Ahmed
Apr 6, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jimena ManzanoBeatriz Morte
Apr 22, 2009·Brain Research·Sheila Cristina de Souza MartinsVivaldo Moura-Neto
Oct 7, 2004·Brain Research. Developmental Brain Research·Juan Ramon Martinez-GalanAntonio Ruiz-Marcos
Apr 1, 2014·General and Comparative Endocrinology·Stitipragyan Bhumika, Veerle M Darras
Mar 17, 2015·Journal of Neurochemistry·Richa KapoorVidita A Vaidya
Jan 7, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A G Trentin, M Alvarez-Silva
Feb 10, 2009·Molecular and Cellular Biochemistry·Jianghong HeChunlin Xia
Dec 13, 2003·Endocrinology·Luis M Garcia-Segura, Margaret M McCarthy
Oct 28, 1999·Endocrinology·A P Farwell, S A Dubord-Tomasetti
Aug 28, 1999·Endocrinology·A P Farwell, S A Dubord-Tomasetti
Jul 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Monique H A KesterGabriella Morreale de Escobar
Jul 17, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F R LimaV Moura Neto
Jul 10, 2003·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Andrew M ArnoldNorman L Eberhardt
Aug 24, 1999·Journal of Neurobiology·I Barakat-Walter
Dec 26, 2001·Journal of Neuroscience Research·Fabienne BuryLouis L Sarliève

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