Thyroid-stimulating antibody assay using a human thyrotropin receptor transfected cell line: relationship to clinical features of graves' disease.

Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists
M R KimM K Gupta

Abstract

To evaluate a Chinese hamster ovary cell line transfected with the human thyrotropin (thyroid-stimulating hormone or TSH) receptor (CHO-TSHR) for its use in routine clinical testing and compare it with the currently used Fisher rat thyroid cell line (FRTL-5) relative to the cyclic adenosine monophosphate (cAMP) responses to thyroid-stimulating antibodies (TSAb). We analyzed 112 serum samples prospectively. TSAb were measured concurrently by both CHO-TSHR cells and FRTL-5 cells. In addition, thyrotropin-binding inhibitory immunoglobulin (TBII) was measured by a radioreceptor assay. Among the study subjects, 51 had active Graves' disease, 40 had other thyroid disease, and 21 had no thyroid disease. Of 51 patients with Graves' disease, 38 (74%) had positive results in both cell assays, 4 (8%) had negative results in both assays, and 9 (18%) had positive responses with CHO-TSHR cells but negative results with FRTL-5 cells. No patient had negative findings with CHO-TSHR cells and positive results by FRTL-5 cells. All 61 patients in the other two groups had negative results by both assays (diagnostic specificity = 100%). The diagnostic sensitivity and accuracy of TSAb activity by the two cell lines (CHO-TSHR versus FRTL-5) were 92.2%...Continue Reading

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Citations

Mar 4, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·M K Gupta
Sep 3, 2010·Autoimmunity Reviews·Simon D Lytton, George J Kahaly
May 18, 2018·Ophthalmic Plastic and Reconstructive Surgery·Tanja Diana, George J Kahaly
Jul 12, 2005·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Israel B OrijaRobert S Zimmerman

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