PMID: 6413633Sep 1, 1983Paper

Thyrotrophin releasing hormone and related peptides in canine tissues

The Journal of Endocrinology
A E PekaryJ M Hershman

Abstract

Thyrotrophin releasing hormone (TRH)-immunoreactive peptides have been quantified in canine serum, hypothalamus, liver, pancreas, adrenal, thyroid, prostate, testis, epididymis and semen by TRH radioimmunoassay, SP-Sephadex C-25 cation exchange chromatography, Sephadex G-10 exclusion chromatography and high pressure liquid chromatography. The total concentration of TRH and TRH-like peptides was highest in the hypothalamus, followed by liver, adrenal, pancreas, thyroid, prostate, epididymis, testis and serum. All of the TRH immunoreactivity (TRH-IR) within extracts of the hypothalamus was due to TRH. On the other hand, nearly all of the TRH-IR of extracts of liver, thyroid, prostate, epididymis, testis and semen was due to TRH-homologous peptides. Adrenal and pancreatic extracts contained a greater proportion of TRH in relation to the TRH-homologous peptides. Extracts of dog serum and semen were found to contain a TRH-binding substance which reduced the retention of added TRH by cation exchangers. The half-time of disappearance (t1/2) of synthetic TRH incubated at 23 degrees C in 10% (w/v) homogenates in 0.15 M-NaCl-0.05 M-phosphate buffer, pH 7.5, ranged from 22 +/- 10 (S.D.) min for liver to 120 +/- 58 min for thyroid. The sho...Continue Reading

Citations

Mar 18, 1999·Pharmacology, Biochemistry, and Behavior·A SattinR L Lloyd
Nov 25, 1998·Thyroid : Official Journal of the American Thyroid Association·A E Pekary
Jun 1, 1990·International Journal of Andrology·A E PekaryS Friedman
Jan 1, 1989·Journal of the American Geriatrics Society·T D Moon, B B Sloane
Aug 27, 2005·Journal of Mass Spectrometry : JMS·A Eugene PekaryAlbert Sattin
Nov 1, 1993·Medical Hypotheses·W E Farnsworth

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