Thyrotropin chronically regulates the pool of thyroperoxidase and its intracellular distribution: a quantitative confocal microscopic study

Journal of Cellular Physiology
C PenelO Chabaud

Abstract

The regulation of thyroperoxidase (TPO) expression and of its intracellular distribution was studied in porcine thyroid cells cultured on porous bottom filters. Cells were cultured for 18 days in the absence or in the presence of thyrotropin (TSH) and with or without iodide. Microsomes were purified and analyzed by electrophoresis. TPO was detected by immunoblotting with polyclonal anti-porcine TPO antibodies and quantified by scanning the bands. The amount of TPO was increased 2-fold by TSH. High concentrations of iodide (1-50 microM, added daily) decreased the level of TPO. Confocal microscopy served to determine the intracellular localization of TPO and its quantitative distribution. Intracellular and surface-located TPO was detected by fluorescein-labeled antibodies on saponin-treated cells. Quantitative confocal microscopy showed that TSH increased the total amount of TPO 2-fold as for immunoblotting. The highest amount of TPO was found in the perinuclear area and between the nucleus and the Golgi apparatus. Only 4% of TPO was present on the apical surface and about 1% on the basolateral membrane; the remainder (about 95%) was inside the cells. TSH did not change these relative contents. TSH modified the intracellular dist...Continue Reading

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Citations

Apr 11, 2006·Annual Review of Nutrition·Michael B Zimmermann
Sep 25, 2004·Environmental Health : a Global Access Science Source·Maureen R GwinnAinsley Weston
Sep 22, 2005·Endocrine Reviews·J KöhrleJ E Dumont
Aug 5, 2000·The Journal of Biological Chemistry·X Zhang, P Arvan
Jul 23, 2005·Endocrinology·Marie-Christine NlendOdile Chabaud
May 27, 2005·The Journal of Biological Chemistry·Regina KuliawatPeter Arvan
Feb 1, 2005·European Journal of Cell Biology·Anton Schmitz, Volker Herzog
Aug 16, 2005·Archives of Biochemistry and Biophysics·Jean Ruf, Pierre Carayon

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