PMID: 221911Apr 1, 1979Paper

Thyrotropin is not a growth factor for human thyroid cells in culture

Proceedings of the National Academy of Sciences of the United States of America
B WestermarkO Wålinder

Abstract

Thyroid cells, obtained from both normal human tissue and benign nodular goiter, were cultured and maintained in vitro in 4-18 passages. Cultures with confluent cells accumulated cyclic AMP (10-150 times the basal amount) upon addition of bovine thyrotropin (100 milliunits/ml), indicating that the cells in culture maintained a thyrotropin-sensitive adenylate cyclase system. Addition of high doses of thyrotropin also induced a characteristic and reversible change in the morphology of the cells. The effect of thyrotropin on cell growth was studied in short- and long-term experiments. Thyrotropin reduced [(3)H]thymidine incorporation in a dose-dependent fashion in all cultures of thyroid cells. The maximal inhibition over a 24-hr period was about 50%. The thyroid cells were notably sensitive, and the half-maximal effect occurred at about 100 milliunits of thyrotropin per ml. In contrast, the hormone had no effect on [(3)H]-thymidine incorporation into human glial cells. Low doses of thyrotropin also had no effect on human fibroblasts and, at high doses, a stimulation of [(3)H]thymidine incorporation was seen. Thyroid cell cultures grown in the presence of 10 milliunits of thyrotropin per ml for 7-14 days had a slower growth rate a...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J Ramachandran, A T Suyama
Jan 1, 1975·Annual Review of Biochemistry·I H PastanW B Anderson
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A L SteinerC Parker
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·H Masui, L D Garren
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R W Holley, J A Kiernan
Jan 1, 1969·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·W R GreigJ S Orr
Feb 1, 1970·Endocrinology·S H Wollman, T R Breitman
Aug 1, 1968·General and Comparative Endocrinology·A Bartke
Sep 15, 1967·International Journal of Cancer. Journal International Du Cancer·J Pontén, E Saksela
Jan 1, 1960·Acta Endocrinologica. Supplementum·P O GEDDA
Jun 1, 1961·Endocrinology·I PASTAN

Citations

Jul 1, 1992·Journal of Cellular Biochemistry·G MilazzoA Belfiore
Jul 1, 1987·Klinische Wochenschrift·G Hintze, J Köbberling
Jul 1, 1994·Clinical & Experimental Metastasis·T HoeltingQ Y Duh
Jan 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J ErrickG N Burrow
Jun 15, 1989·Biochimica Et Biophysica Acta·N TakasuT Yamada
Sep 1, 1982·Molecular and Cellular Endocrinology·F A KarlssonB Westermark
Jun 1, 1985·Molecular and Cellular Endocrinology·N E HeldinB Westermark
Mar 1, 1986·Molecular and Cellular Endocrinology·W H Dere, B Rapoport
May 1, 1986·Molecular and Cellular Endocrinology·S HovsépianG Fayet
May 1, 1987·Molecular and Cellular Endocrinology·D W WilliamsE D Williams
May 1, 1989·Molecular and Cellular Endocrinology·L del SennoG Trasforini
Apr 1, 1991·Molecular and Cellular Endocrinology·J P BergE Haug
Feb 5, 1996·Molecular and Cellular Endocrinology·A K RasmussenU Feldt-Rasmussen
Aug 1, 1988·Baillière's Clinical Endocrinology and Metabolism·G A Thomas, E D Williams
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J YangS Nandi
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L Nitsch, S H Wollman
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F S Ambesi-ImpiombatoH G Coon
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T IguchiH A Bern
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·F CurcioH G Coon
Jul 1, 1982·The Journal of Cell Biology·B Westermark, K R Portor
Oct 3, 2000·Thyroid : Official Journal of the American Thyroid Association·J D BergströmN E Heldin
Aug 24, 2002·Thyroid : Official Journal of the American Thyroid Association·G Fayet, S Hovsépian
Jan 1, 1992·Thyroid : Official Journal of the American Thyroid Association·Y C TsengL Wartofsky
Oct 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G O NessJ R Lillehaug
Dec 1, 1994·Journal of Endocrinological Investigation·D Tramontano, G Villone
May 1, 1990·Journal of Endocrinological Investigation·G DamanteS Filetti
Apr 1, 1985·Molecular and Cellular Endocrinology·K WestermarkB Westermark
Dec 1, 1991·Baillière's Clinical Endocrinology and Metabolism·J E DumontP P Roger
Dec 1, 1992·Experimental Cell Research·H KimuraS Nagataki
Jun 1, 1984·Medical Hypotheses·A LewińskiR J Reiter
Apr 1, 1988·Clinical Endocrinology·S P Bidey, S Tomlinson
Aug 15, 1988·Biochemical and Biophysical Research Communications·L K BachrachG N Burrow
Dec 16, 1987·Biochemical and Biophysical Research Communications·P P Roger, J E Dumont
Jun 2, 2015·PloS One·Valeria LucciMariastella Zannini

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.