Thyrotropin receptors in normal and pathological human thyroid tissues

The Journal of Clinical Endocrinology and Metabolism
H TakahashiC Y Lee

Abstract

The properties of TSH receptors in normal and pathological human thyroid tissues were studied. Highly purified bovine TSH after lactoperoxidase iodination retained full biological activity, as assessed by radioreceptor assay. Binding of bovine [125I]TSH to 1000 x g pellets of human thyroid homogenate was specific with respect to hormone and tissue. Total binding amounted to 50-60% of total radioactivity using 10 mg (wet weight) normal thyroid tissue. Nonspecific binding was only 6% of total radioactivity. Normal thyroid tissue contained two orders of binding sites, which were shown to be independent of each other by Hill plot analysis. The high affinity sites [equilibrium dissociation constant (Kd), 0.015-0.16 x 10-9 M] were present in concentrations of 1.05-9.30 pmol/mg protein and concentrations of low affinity sites (Kd, 1.2-2.4 x 10-9 M) were 35.9-213 pmol/mg. In all pathological thyroid tissue studied, two orders of binding sites were found with dissociation constants similar to those of normal tissues, but the number of binding sites was markedly reduced. Both orders of binding sites in solitary "cold" adenomas and only the low affinity sites in thyroid tissue from patients with Graves' disease were significantly reduced ...Continue Reading

References

Apr 1, 1997·Thyroid : Official Journal of the American Thyroid Association·J C Morris
Apr 10, 2009·Thyroid : Official Journal of the American Thyroid Association·Thomas RinkFrank Grünwald
Apr 29, 1998·The Journal of Clinical Investigation·T SaitoT Onaya
Jan 1, 1981·World Journal of Surgery·O H Clark
Nov 1, 1980·Pathology, Research and Practice·F Hofstädter
Mar 1, 1988·Journal of Endocrinological Investigation·H W Müller-GärtnerV Bay
Jul 1, 1980·Journal of Endocrinological Investigation·M A Pisarev, D L Kleiman de Pisarev
Jun 1, 1990·Endocrine Pathology·Virginia A LiVoisi

Related Concepts

Adenoma, Trabecular
Hormone Receptors, Cell Surface
Carcinomatosis
LPO
Medullary Carcinoma
Adenoma
Centrifugation
Thyroid Diseases
Thyrotropin
Carcinoma, Cribriform

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