Thyrotropin-releasing hormone-stimulated thyrotropin expression involves islet-brain-1/c-Jun N-terminal kinase interacting protein-1

Endocrinology
Hiroshi AbeToshihiko Ishida

Abstract

Islet-brain-1 (IB1)/c-Jun N-terminal kinase interacting protein 1 (JIP-1) is a scaffold protein that is expressed at high levels in neurons and the endocrine pancreas. IB1/JIP-1 interacts with the c-Jun N-terminal kinase and mediates the specific physiological stimuli (such as cytokines). However, the potential role of the protein in the pituitary has not been evaluated. Herein, we examined expression of the gene encoding IB1/JIP-1 and its translated product in the anterior pituitary gland and a pituitary cell line, GH3. We then examined the potential role of IB1/JIP-1 in controlling TSH-beta gene expression. Exposure of GH3 cells to TRH stimulated the expression of IB1/JIP-1 protein levels, mRNA, and transcription of the promoter. The increase of IB1/JIP-1 content by transient transfection study of a vector encoding IB1/JIP-1 or by the stimulation of TRH stimulates TSH-beta promoter activity. This effect is not found in the presence of a mutated nonfunctional (IB1S59N) IB1/JIP-1 protein. Together, these facts point to a central role of the IB1/JIP-1 protein in the control of TRH-mediated TSH-beta stimulation.

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Citations

Oct 13, 1988·Biochimica Et Biophysica Acta·J L Chastain, D B McCormick
Apr 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Andrew Nguyen, Hua Cai
Dec 28, 2010·Canadian Journal of Physiology and Pharmacology·Mohammad KhaksariGholamreza Asadikaram
Mar 10, 2009·Journal of Cellular and Molecular Medicine·Tomie MuraokaToshihiko Ishida

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