Thyroxine 5'-deiodination mediates norepinephrine-induced lipogenesis in dispersed brown adipocytes

Endocrinology
A C BiancoA S Moriscot

Abstract

In euthyroid rats, maximal sympathetic nervous system stimulation (e.g. during cold exposure) results in a 3- to 4-fold increase in brown adipose tissue lipogenesis, a response that is blunted in hypothyroid rats. To further investigate this phenomenon, the role of local type II 5'-deiodinase (5'-DII) was studied in freshly isolated brown adipocytes. In a typical experiment, 1.5 x 10(6) cells were incubated for up to 48 h in a water-saturated 5% CO2-95% O2 atmosphere. After incubation with medium alone or with different concentrations of T4, T3, and/or norepinephrine (NE), lipogenesis was studied by measuring 1) the rate of fatty acid synthesis as reflected by 3H2O incorporation into lipids and 2) the activity of key rate-limiting enzymes, i.e. acetyl coenzyme A carboxylase and malic enzyme, and the results are reported in terms of DNA content per tube. Lipogenesis decreased progressively over time (approximately 40%) when no additions were made to the incubation medium. T4 or T3 partially prevented that inhibition at physiological concentrations (65 x 10[-9] and 0.77 x 10[-9] M, respectively), whereas a receptor-saturating concentration of T3, (154 x 10[-9] M) doubled the lipogenesis rate. The addition of 10(-6) M NE inhibited...Continue Reading

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Citations

Nov 7, 2001·The Journal of Clinical Investigation·L A de JesusA C Bianco
Mar 29, 2006·Clinics in Laboratory Medicine·Daniel E Rusyniak, Jon E Sprague
Nov 11, 2011·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·A Sainsbury, L Zhang
Oct 18, 2005·The Medical Clinics of North America·Daniel E Rusyniak, Jon E Sprague
Feb 11, 2015·Molecular and Cellular Endocrinology·Raquel Martinez-deMenaMaria-Jesus Obregon
Mar 15, 2011·Metabolism: Clinical and Experimental·Rosa Maria Calvo, Maria-Jesus Obregon
Dec 12, 2012·Biochimica Et Biophysica Acta·Alexander BarteltLudger Scheja
Nov 12, 2005·Bioscience Reports·Antonio C BiancoMarcelo A Christoffolete
Apr 30, 2019·Endocrine Reviews·Antonio C BiancoBarbara M L C Bocco
Oct 9, 2014·American Journal of Physiology. Endocrinology and Metabolism·Ruy A LouzadaDenise P Carvalho
Nov 26, 2010·Expert Review of Cardiovascular Therapy·Stella StabouliVasilios Kotsis
Oct 17, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F A C SearaE L Olivares
Apr 30, 2020·International Journal of Molecular Sciences·Winifred W Yau, Paul M Yen
Sep 26, 2008·Endocrine Reviews·Balázs GerebenAntonio C Bianco
Jan 8, 2015·Frontiers in Physiology·Maria-Jesus Obregon
Sep 27, 2018·Frontiers in Endocrinology·Annunziata G CicatielloMonica Dentice
Jan 14, 1999·The American Journal of Physiology·M BrancoA C Bianco
Mar 16, 2021·Endocrinology·Samuel C RussoAntonio C Bianco

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