PMID: 3751464Aug 1, 1986Paper

Thyroxine monodeiodination in normal human kidney tissue in vitro

Acta Endocrinologica
N Boye

Abstract

The present study deals with thyroxine monodeiodination in normal human kidney. To allow for comparison with previous reports, the present methods are similar to those used by others in rat tissue studies. The microsomal cell fraction of normal human kidney tissue was obtained by differential ultracentrifugation. The microsomes were incubated under various conditions and the deiodination products assayed with radioimmunoassay. A type I 5'-monodeiodinase was demonstrated, pH optimum around 6.5. Competitive inhibition was observed of T3 generation from T4 by rT3 with a Km of 3.0 microM and a Ki of 4 microM. Vmax was 26.1 pmol/min/mg protein. Likewise rT3 was generated from added T4, but it was rapidly degraded, while T3 was relatively stable as is the case in rat tissue preparations. Propylthiouracil inhibited 5'-deiodination in a dose dependent fashion with complete abolishment of deiodination at propylthiouracil concentration of 10(-4) M. Ipodate inhibited the reaction with complete inhibition at 10(-2) M. The data demonstrate that a human kidney particulate cell-fraction contained considerable amounts of T4 deiodinases, very similar to the type I deiodinase of various rat tissue, although the handling of rT3 and the inhibitory...Continue Reading

Citations

Mar 1, 1988·Molecular and Cellular Endocrinology·N BoyeP L Jørgensen
Oct 23, 1998·Molecular and Cellular Endocrinology·M R StulpC Ris-Stalpers
Jun 1, 2001·Journal of Endocrinological Investigation·J PachuckiA Nauman
Aug 30, 2001·Thyroid : Official Journal of the American Thyroid Association·L SabatinoG Iervasi
Jun 27, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hiroyuki YamanakaTsuyoshi Yokoi
Dec 1, 1989·Metabolism: Clinical and Experimental·J K LeeM M Kaplan

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