Tiazofurin induces a down-modulation of ICAM-1 expression on K562 target cells impairing NK adhesion and killing

Cellular Immunology
L ZamaiM Vitale

Abstract

Tiazofurin treatment of K562 leukemia cells in vitro depletes the metabolites of the guanylate biosynthetic pathway, inducing erythroid differentiation, that, in turn, alters the phenotypic profile. As a consequence, K562 cells possibly modify their interaction with immune cells. Here we describe the binding and killing activity of peripheral blood NK cells against differentiating K562 cells and the correlation between their altered binding capacity and ICAM-1 expression levels in differentiating K562 cells. We found that decreased percentages of NK (and T) cells were bound to differentiating K562 cells generating a decreased cytotoxic activity. This corresponded to decreased expression of ICAM-1, as detected by FACS analysis and Western blot. Erythroid differentiation, binding and killing reduction, and ICAM-1 down-modulation were completely abrogated by guanosine treatment. Tiazofurin causes a decrease in lymphocyte recognition and binding to K562 target cells. This can be ascribed to the down-modulation of ICAM-1 expression on target cells, which, therefore, can escape killing, acquiring a selective survival advantage.

Citations

Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·O AyalonJ R Bender
Jun 11, 1997·International Journal of Cancer. Journal International Du Cancer·A C BudinskyC C Zielinski
Jan 19, 1999·The Anatomical Record·A R MarianiM Vitale
Jul 13, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Tillmann CyrusGregory M Lanza
Jul 19, 2011·IUBMB Life·Ronald Carnemolla, Vladimir R Muzykantov
Dec 17, 2020·Cancers·Mieszko LachotaKarl-Johan Malmberg

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