Tick-borne encephalitis virus envelope protein E-specific monoclonal antibodies for the study of low pH-induced conformational changes and immature virions

Archives of Virology
Heidemarie HolzmannF X Heinz

Abstract

A set of ten monoconal antibodies (mabs) specific for the tick-borne encephalitis (TBE) virus envelope protein E were prepared and characterized with respect to their functional activities, the location of their binding sites on protein E and the involvement of their epitopes in acid pH-induced conformational changes and interactions with the precursor to the membrane protein (prM) in immature virions. The majority of these mabs mapped to the previously defined antigenic domain A. All of the mabs recognize parts of the E protein which undergo low pH-induced structural rearrangements believed to be necessary for the fusion activity of the virus, and six of the mabs define epitopes which are affected by the prM-E interaction in immature virions. They are therefore of potential value as specific reagents for studying the structure and function of protein E, as well as the function of the prM-E association. Five of the mabs exhibited neutralizing activity, and can therefore be used for the selection of escape mutants.

References

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Citations

May 13, 2008·Expert Reviews in Molecular Medicine·Theodore C Pierson, Michael S Diamond
Sep 16, 2006·Journal of Virology·Karin StiasnyFranz X Heinz
Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Regina M KoflerChristian W Mandl
Mar 27, 2012·Current Opinion in Virology·Theodore C Pierson, Michael S Diamond
Sep 16, 2017·PLoS Pathogens·Denise HaslwanterKarin Stiasny

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