Tick-Borne Encephalitis Virus Infection Alters the Sialome of Ixodes ricinus Ticks During the Earliest Stages of Feeding.

Frontiers in Cellular and Infection Microbiology
Charles E HartSaravanan Thangamani

Abstract

Ticks are hematophagous arthropods that transmit a number of pathogens while feeding. Among these is tick-borne encephalitis virus (TBEV), a flavivirus transmitted by Ixodes ricinus ticks in the temperate zone of Europe. The infection results in febrile illness progressing to encephalitis and meningitis with a possibility of fatality or long-term neurological sequelae. The composition of tick saliva plays an essential role in the initial virus transmission during tick feeding. Ticks secrete a diverse range of salivary proteins to modulate the host response, such as lipocalins to control the itch and inflammatory response, and both proteases and protease inhibitors to prevent blood coagulation. Here, the effect of viral infection of adult females of Ixodes ricinus was studied with the goal of determining how the virus alters the tick sialome to modulate host tissue response at the site of infection. Uninfected ticks or those infected with TBEV were fed on mice and removed and dissected one- and 3-h post-attachment. RNA from the salivary glands of these ticks, as well as from unfed ticks, was extracted and subjected to next-generation sequencing to determine the expression of key secreted proteins at each timepoint. Genes showing...Continue Reading

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Citations

Dec 29, 2020·Parasite Immunology·Charles Edward Hart, Saravanan Thangamani
Mar 7, 2021·International Journal of Environmental Research and Public Health·Michael PhamAdela S Oliva Chávez

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Datasets Mentioned

BETA
SRR10240050
GHXN00000000

Methods Mentioned

BETA
Dissection
RNA-Seq

Software Mentioned

blast
rpsblast
MEGA7
Trinity
Abyss
fasta
blastn
R package
R R

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