Tick-borne encephalitis virus (TBEV) prevalence in field-collected ticks (Ixodes ricinus) and phylogenetic, structural and virulence analysis in a TBE high-risk endemic area in southwestern Germany

Parasites & Vectors
Daniela OttThorsten Lenhard

Abstract

Tick-borne encephalitis (TBE) is the most common viral CNS infection with incidences much higher than all other virus infections together in many risk areas of central and eastern Europe. The Odenwald Hill region (OWH) in southwestern Germany is classified as a TBE risk region and frequent case numbers but also more severe infections have been reported within the past decade. The objective of the present study was to survey the prevalence of tick-borne encephalitis virus (TBEV) in Ixodes ricinus and to associate TBEV genetic findings with TBE infections in the OWH. Ticks were collected by the flagging methods supported by a crowdsourcing project implementing the interested public as collectors to cover completely and collect randomly a 3532 km2 area of the OWH TBE risk region. Prevalence of TBEV in I. ricinus was analysed by reversed transcription quantitative real-time PCR. Phylogeographic analysis was performed to classify OWH TBEV isolates within a European network of known TBEV strains. Mutational sequence analysis including 3D modelling of envelope protein pE was performed and based on a clinical database, a spatial association of TBE case frequency and severity was undertaken. Using the crowd sourcing approach we could an...Continue Reading

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Datasets Mentioned

BETA
KF056331
U27495
U39292

Methods Mentioned

BETA
electrophoresis
PCR
X-ray
amino acid exchange
nucleotide exchange

Software Mentioned

Molecular Graphic and 3D Viewer
HHsuite
Clustal
BEAST
Google Maps
SPLITS TREE
HHsuite ToolKit
SumTrees
DendroPy Phylogenetic Computing Library
Clustal Omega

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