Tick galactosyltransferases are involved in α-Gal synthesis and play a role during Anaplasma phagocytophilum infection and Ixodes scapularis tick vector development.

Scientific Reports
Alejandro Cabezas-CruzJosé de la Fuente

Abstract

The carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) is produced in all mammals except for humans, apes and old world monkeys that lost the ability to synthetize this carbohydrate. Therefore, humans can produce high antibody titers against α-Gal. Anti-α-Gal IgE antibodies have been associated with tick-induced allergy (i.e. α-Gal syndrome) and anti-α-Gal IgG/IgM antibodies may be involved in protection against malaria, leishmaniasis and Chagas disease. The α-Gal on tick salivary proteins plays an important role in the etiology of the α-Gal syndrome. However, whether ticks are able to produce endogenous α-Gal remains currently unknown. In this study, the Ixodes scapularis genome was searched for galactosyltransferases and three genes were identified as potentially involved in the synthesis of α-Gal. Heterologous gene expression in α-Gal-negative cells and gene knockdown in ticks confirmed that these genes were involved in α-Gal synthesis and are essential for tick feeding. Furthermore, these genes were shown to play an important role in tick-pathogen interactions. Results suggested that tick cells increased α-Gal levels in response to Anaplasma phagocytophilum infection to control bacterial infection. These results provided the mo...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1996·Methods in Enzymology·T L MaddenJ Zhang
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 10, 1998·Molecular Microbiology·M P JenningsE R Moxon
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Sep 12, 2002·Cellular and Molecular Life Sciences : CMLS·T Hennet
Apr 1, 2004·Journal of Comparative Pathology·L Bell-Sakyi
Jun 25, 2004·Nucleic Acids Research·David E KimDavid Baker
Feb 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie MillandMauro S Sandrin
Jan 24, 2007·Glycobiology·Joseph R Bishop, Pascal Gagneux
Jul 28, 2007·Trends in Parasitology·José de la FuenteEdmour F Blouin
Jul 31, 2007·Trends in Parasitology·Lesley Bell-SakyiFrans Jongejan
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Mar 28, 2008·Molecular Biology and Evolution·Si Quang Le, Olivier Gascuel
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Jose de la FuenteDaniel E Sonenshine
Jul 29, 2010·The Journal of Clinical Investigation·Takeshi WadaHajime Karasuyama
Apr 2, 2011·The Journal of Allergy and Clinical Immunology·Scott P ComminsThomas A E Platts-Mills
Jan 8, 2013·Vaccine·Juan A Moreno-CidUNKNOWN SUB/AKR Vaccine Study Group
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Mar 26, 2013·Case Reports in Dermatology·Kyoko NakahigashiKenji Kabashima
Jul 26, 2013·Frontiers in Cellular and Infection Microbiology·Snorre StuenCornelia Silaghi
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Nov 22, 2014·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Mar 10, 2015·The Journal of Allergy and Clinical Immunology·John W SteinkeScott P Commins
Jul 2, 2015·Current Allergy and Asthma Reports·Thomas A E Platts-MillsScott P Commins

❮ Previous
Next ❯

Citations

Mar 8, 2019·Current Opinion in Allergy and Clinical Immunology·Jeffrey M Wilson, Thomas A E Platts-Mills
Sep 22, 2019·Vaccines·Adnan HodžićGeorg Gerhard Duscher
Jun 17, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Krzyszof RutkowskiAnna Moniuszko-Malinowska
Jul 7, 2020·Expert Review of Molecular Diagnostics·José de la FuenteIván Pacheco
Apr 5, 2019·Parasites & Vectors·José de la FuenteAlejandro Cabezas-Cruz
May 16, 2019·Wiener klinische Wochenschrift·Veronika PisazkaFranz Allerberger
Aug 9, 2020·Frontiers in Immunology·Jessica L ChandrasekharLoren D Erickson
Aug 2, 2019·Experimental & Applied Acarology·José Francisco Lima-BarberoJosé de la Fuente
Jun 20, 2019·Frontiers in Immunology·Alejandro Cabezas-CruzJosé de la Fuente
Nov 26, 2020·Vaccines·Lourdes Mateos-HernándezAlejandro Cabezas-Cruz
Nov 14, 2019·Allergologia et immunopathologia·J Martín-LázaroF Carballada
Mar 18, 2020·The World Allergy Organization Journal·UNKNOWN Steering Committee Authors, UNKNOWN Review Panel Members
Oct 1, 2019·The Journal of Allergy and Clinical Immunology. in Practice·Thomas A E Platts-MillsJeffrey M Wilson
Sep 20, 2020·Trends in Parasitology·Adnan HodžićAlejandro Cabezas-Cruz
May 15, 2021·The Biochemical Journal·Alejandro Cabezas-CruzJosé de la Fuente
Jul 1, 2021·Pathogens and Global Health·Cristiano SalataLesley Bell-Sakyi
Aug 7, 2021·Frontiers in Cellular and Infection Microbiology·Surendra Raj Sharma, Shahid Karim
Aug 2, 2021·The Journal of Allergy and Clinical Immunology·Tatenda MurangiMichael E Levin
Dec 6, 2019·International Archives of Allergy and Immunology·Jörg FischerAmir Sadegh Yazdi
Dec 21, 2021·Frontiers in Cellular and Infection Microbiology·Surendra Raj SharmaShahid Karim

❮ Previous
Next ❯

Datasets Mentioned

BETA
PXD002181

Methods Mentioned

BETA
glycosylation
flow cytometry
gene knockdown
ELISA
transfection
flow
galactotransferases
PCR
affinity column chromatography
electrophoresis

Software Mentioned

Molecular Evolutionary Genetics Analysis ( MEGA )
DeepLoc
MAFFT
MEGA
CellQuest Pro
BLAST
Robetta
Gblocks
TASSER
genNorm

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.