Tick vitellin is dephosphorylated by a protein tyrosine phosphatase during egg development: effect of dephosphorylation on VT proteolysis

Insect Biochemistry and Molecular Biology
Alan B SilveiraMário A C Silva-Neto

Abstract

Vitellin (VT) is a phospholipoglycoprotein that is the main component of arthropod egg yolk. Phosphorylation is a recurrent feature of every VT molecule described so far. However, the role played by such post-translational modification during egg development is not yet clear. In the eggs of the hard tick Boophilus microplus, VT is a phosphotyrosine-containing protein. VT-phosphotyrosine residues are gradually removed during tick embryogenesis due to the action of a 45 kDa egg tyrosine phosphatase. This enzyme is strongly inhibited by ammonium molybdate, sodium vanadate and cupric ion. The role of phosphotyrosine residues in VT proteolytic degradation was evaluated. Western blots probed with a monoclonal anti-phosphotyrosine antibody demonstrated that the high molecular mass VT subunits (VT 1 and VT 2) are the main targets of dephosphorylation during egg development. Both dephosphorylation and proteolysis of VT 1 and VT 2 are blocked by ammonium molybdate in total egg homogenates. When purified VT was dephosphorylated in vitro with lambda phosphatase and then incubated in the presence of bovine cathepsin D, VT proteolysis increased dramatically. Altogether, these data are the first to show that phosphotyrosine residues are prese...Continue Reading

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Citations

Jul 18, 2014·PLoS Neglected Tropical Diseases·André L Costa-da-SilvaMargareth L Capurro
Sep 11, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Paula C PohlAoi Masuda
Aug 19, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Eldo CamposCarlos Logullo
Sep 6, 2007·Insect Biochemistry and Molecular Biology·Eldo CamposCarlos Logullo
Sep 7, 2011·Memórias do Instituto Oswaldo Cruz·Willy JablonkaMário Alberto C Silva-Neto
Apr 7, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·S RomeroM Cunningham

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