PMID: 7031642Oct 1, 1981Paper

tif-1 mutation alters polynucleotide recognition by the recA protein of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
K McEntee, G M Weinstock

Abstract

The requirements for polynucleotide-dependent hydrolysis of ATP and for proteolytic cleavage of phage lambda repressor have been examined for both the wild-type (recA+ protein) and the tif-1 mutant form [tif(recA) protein] of the recA gene product. The recA+ and tif(recA) proteins catalyze both reactions in the presence of long single-stranded DNAs or certain deoxyhomopolymers. However, short oligonucleotides [(dT)12, (dA)14] stimulate neither the protease nor the ATPase activities of the recA+ protein. In contrast, these short oligonucleotides activate tif(recA) protein to cleave lambda repressor without stimulating its ATPase activity. Moreover, both the ATPase and protease activities of the tif(recA) protein are stimulated by poly(rU) and poly(rC) whereas the recA+ protein does not respond to these ribopolymers. We have purified the recA protein from a strain in which the tif mutation is intragenically suppressed. This mutant protein (recA629) is inactive in the presence of (dT)12, (dA)14, poly(rU), and poly(rC) for lambda repressor cleavage and ATP hydrolysis. These results argue that the tif-1 mutation (or mutations) alters the DNA binding site of the recA protein. We suggest that in vivo the tif(recA) protein is activated...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T ShibataC M Radding
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·T OgawaH Ogawa
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D W Mount
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K McEntee
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J W RobertsN L Craig
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G M WeinstockI R Lehman
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K McEnteeI R Lehman
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J W Roberts, C W Roberts
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J W LittleD W Mount
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Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R M IrbeM Oishi

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Citations

Nov 1, 1988·Molecular & General Genetics : MGG·A BailoneR Devoret
Jan 1, 1984·Molecular & General Genetics : MGG·H KawashimaH Ogawa
Aug 1, 1982·Biochimie·M BlancoL M Botella
May 1, 1982·Cell·J W Little, D W Mount
Feb 12, 1999·Journal of Molecular Biology·M J Campbell, R W Davis
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·Z LivnehT Elizur
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·A I Roca, M M Cox
Apr 1, 2015·Nucleic Acids Research·Katarzyna H MaslowskaRoel M Schaaper
Nov 18, 2018·Environmental and Molecular Mutagenesis·Katarzyna H MaslowskaIwona J Fijalkowska
Oct 1, 1990·Journal of Bacteriology·S K Liu, I Tessman
Jun 1, 1990·Journal of Bacteriology·J B SweasyV Roegner-Maniscalco

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