Abstract
Our previous study showed that TP53-induced glycolysis and apoptosis regulator (TIGAR) regulated ROS, autophagy, and apoptosis in response to hypoxia and chemotherapeutic drugs. Aescin, a triterpene saponin, exerts anticancer effects and increases ROS levels. The ROS is a key upstream signaling to activate autophagy. Whether there is a crosstalk between TIGAR and aescin in regulating ROS, autophagy, and apoptosis is unknown. In this study, we found that aescin inhibited cell viability and colony formation, and induced DNA damage, cell cycle arrest, and apoptosis in cancer cell lines HCT-116 and HCT-8 cells. Concurrently, aescin increased the expression of TIGAR, ROS levels, and autophagy activation. Knockdown of TIGAR enhanced the anticancer effects of aescin in vitro and in vivo, whereas overexpression of TIGAR or replenishing TIGAR downstream products, NADPH and ribose, attenuated aescin-induced apoptosis. Furthermore, aescin-induced ROS elevation and autophagy activation were further strengthened by TIGAR knockdown in HCT-116 cells. However, autophagy inhibition by knockdown of autophagy-related gene ATG5 or 3-methyladenine (3-MA) exaggerated aescin-induced apoptosis when TIGAR was knocked down. In conclusion, TIGAR plays a ...Continue Reading
References
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P O Seglen, P B Gordon
Jul 5, 2006·Molecular Cancer Therapeutics·Jagan M R PatlollaChinthalapally V Rao
Jul 15, 2006·Cell·Karim BensaadKaren H Vousden
Aug 11, 2006·Trends in Molecular Medicine·Wynand P Roos, Bernd Kaina
Aug 29, 2009·The EMBO Journal·Karim BensaadKaren H Vousden
Oct 23, 2009·Nature·Stephen P Jackson, Jiri Bartek
Dec 19, 2009·The New England Journal of Medicine·Sanford D Markowitz, Monica M Bertagnolli
Jan 28, 2010·Molecular Pharmacology·Kuzhuvelil B HarikumarBharat B Aggarwal
Jan 29, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Z J MingX G Zhang
Apr 10, 2010·The Journal of Pharmacology and Experimental Therapeutics·Sandra Min-Li TanGautam Sethi
Oct 23, 2010·Molecular Cell·Guido KroemerBeth Levine
Nov 26, 2010·Annual Review of Pathology·Eric R Fearon
Mar 15, 2011·Molecular Carcinogenesis·Deng Bo JiJing Rong Cui
Aug 9, 2011·Human Pathology·Kyu Yeoun WonDong-Ki Lee
Nov 15, 2011·Cell·Noboru Mizushima, Masaaki Komatsu
Jan 25, 2012·Journal of Cancer Research and Clinical Oncology·Yong-Wei WangBei Sun
Apr 27, 2012·Nature Reviews. Cancer·Eileen White
Aug 14, 2012·The Journal of Biological Chemistry·Christina WankaJohannes Rieger
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eric C CheungKaren H Vousden
Jun 4, 2013·Developmental Cell·Eric C CheungKaren H Vousden
Sep 26, 2013·Nature Reviews. Cancer·Caitriona HolohanPatrick G Johnston
Nov 15, 2013·Lancet·Hermann BrennerChristian Peter Pox
Feb 11, 2014·Free Radical Biology & Medicine·Haowen ZhangFenju Liu
Jun 10, 2014·Nutrition and Cancer·Hyun Sook LeeSun Hyo Kim
Aug 3, 2014·Cancer Research·Jia-Ming XieZheng-Hong Qin
Jan 27, 2015·Oncology Letters·Elaine Yue Ling WongAnthony Tak Cheung Chan
Jan 30, 2015·World Journal of Gastroenterology : WJG·Gui-Li HuangQing-Xi Chen
May 1, 2015·Scientific Reports·Hong-Pei YuZheng-Hong Qin
Aug 8, 2015·Cell Death & Disease·P LeeE C Cheung
Aug 12, 2015·Cancer Letters·Liqiang QianChangqing Su
Oct 11, 2015·Free Radical Biology & Medicine·Lu ZhangCanhua Huang
Dec 18, 2015·Oncology Reports·Khayal Al-KhayalRehan Ahmad
Jan 30, 2016·Acta biochimica Polonica·Gabriela MojžišováJán Mojžiš
Mar 24, 2016·Oncotarget·Yuhui WangYue Dai
Nov 26, 2016·Journal of Hematology & Oncology·Sixuan QianYaoyu Chen
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Mar 2, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jun 13, 2017·Acta Pharmacologica Sinica·Li-Mei FangZheng-Hong Qin
Citations
Jul 2, 2020·OncoTargets and Therapy·Jin-Ling ZhouGang Liang
Nov 20, 2018·Frontiers in Oncology·Weifen ZhangWentong Li
Nov 26, 2020·Medicine·Li ZhouXuewei Gu
Feb 5, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Bing Han, Chengwei He
Feb 15, 2020·Toxicology·Zi-Qi LiuYan Wang
Jul 1, 2020·Recent Patents on Anti-cancer Drug Discovery·Javad Saffari-ChaleshtoriSayed Mohammad Shafiee
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Avez SharipovJamoliddin Razzokov