TIGAR promotes neural stem cell differentiation through acetyl-CoA-mediated histone acetylation

Cell Death & Disease
Wenjuan ZhouAijun Hao

Abstract

Cellular metabolism plays a crucial role in controlling the proliferation, differentiation, and quiescence of neural stem cells (NSCs). The metabolic transition from aerobic glycolysis to oxidative phosphorylation has been regarded as a hallmark of neuronal differentiation. Understanding what triggers metabolism reprogramming and how glucose metabolism directs NSC differentiation may provide new insight into the regenerative potential of the brain. TP53 inducible glycolysis and apoptosis regulator (TIGAR) is an endogenous inhibitor of glycolysis and is highly expressed in mature neurons. However, its function in embryonic NSCs has not yet been explored. In this study, we aimed to investigate the precise roles of TIGAR in NSCs and the possible involvement of metabolic reprogramming in the TIGAR regulatory network. We observed that TIGAR is significantly increased during brain development as neural differentiation proceeds, especially at the peak of NSC differentiation (E14.5-E16.5). In cultured NSCs, knockdown of TIGAR reduced the expression of microtubule-associated protein 2 (MAP2), neuron-specific class III beta-tubulin (Tuj1), glial fibrillary acidic protein (GFAP), Ngn1, and NeuroD1, and enhanced the expression of REST, sug...Continue Reading

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Citations

Aug 29, 2019·Frontiers in Molecular Neuroscience·Wenjuan ZhouLiang Kong
Jan 9, 2020·Cellular and Molecular Life Sciences : CMLS·Camilla MaffezziniChristoph Freyer
Jan 30, 2021·Acta Pharmacologica Sinica·Jie TangRui Sheng
Mar 6, 2021·Cell Stem Cell·Ram Prosad Chakrabarty, Navdeep S Chandel
Oct 24, 2020·Trends in Genetics : TIG·Richard W Carthew
Apr 17, 2021·Free Radical Biology & Medicine·Inah HwangJihye Paik
Jun 26, 2021·Current Opinion in Neurobiology·Ryohei Iwata, Pierre Vanderhaeghen
Aug 5, 2021·Disease Models & Mechanisms·Larissa TraxlerJerome Mertens
Oct 19, 2021·Frontiers in Pharmacology·Lijun YangQinjie Weng

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Methods Mentioned

BETA
PCR
fluorescence microscopy
immunoprecipitation
histone acetylation
acetylation
ChIP

Software Mentioned

SPSS
ImageJ
NIH ImageJ

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