Tigecycline resistance in clinical isolates of Enterococcus faecium is mediated by an upregulation of plasmid-encoded tetracycline determinants tet(L) and tet(M)

The Journal of Antimicrobial Chemotherapy
S FiedlerGuido Werner

Abstract

Tigecycline represents one of the last-line therapeutics to combat multidrug-resistant bacterial pathogens, including VRE and MRSA. The German National Reference Centre for Staphylococci and Enterococci has received 73 tigecycline-resistant Enterococcus faecium and Enterococcus faecalis isolates in recent years. The precise mechanism of how enterococci become resistant to tigecycline remains undetermined. This study documents an analysis of the role of efflux pumps in tigecycline resistance in clinical isolates of Enterococcus spp. Various tigecycline MICs were found for the different isolates analysed. Tigecycline-resistant strains were analysed with respect to genome and transcriptome differences by means of WGS and RT-qPCR. Genes of interest were cloned and expressed in Listeria monocytogenes for verification of their functionality. Detailed comparative whole-genome analyses of three isogenic strains, showing different levels of tigecycline resistance, revealed the major facilitator superfamily (MFS) efflux pump TetL and the ribosomal protection protein TetM as possible drug resistance proteins. Subsequent RT-qPCR confirmed up-regulation of the respective genes. A correlation of gene copy number and level of MIC was inferred...Continue Reading

References

Oct 1, 1987·Antimicrobial Agents and Chemotherapy·L M McMurryS B Levy
Jul 22, 1998·FEMS Microbiology Letters·S Song, C Park
Jun 27, 2000·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·G WernerW Witte
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·I Chopra, M Roberts
Oct 27, 2001·Science·P GlaserP Cossart
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·Charles R DeanPatricia A Bradford
May 25, 2004·Antimicrobial Agents and Chemotherapy·Andrea Sudano RoccaroVincenzo Enea
Apr 20, 2005·FEMS Microbiology Letters·Marilyn C Roberts
Jun 14, 2005·Biochimie·Béatrice Marquez
Sep 1, 2005·Biochemistry·Ian F MooreGerard D Wright
May 26, 2006·Antimicrobial Agents and Chemotherapy·Matthew W OlsonPatricia A Bradford
Feb 5, 2008·Bioorganic & Medicinal Chemistry Letters·Nadezhda GermanRobert J Kerns
Feb 21, 2008·The Journal of Antimicrobial Chemotherapy·Guido WernerIngo Klare
Mar 25, 2008·European Journal of Medicinal Chemistry·Nadezhda GermanRobert J Kerns
Apr 29, 2008·Applied and Environmental Microbiology·Ian R MonkColin Hill
Oct 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yuriy MileykoJoshua S Weitz
Mar 20, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M KreskenC von Eiff
Nov 3, 2009·Journal of Microbiological Methods·L B JensenF M Aarestrup
Oct 19, 2010·International Journal of Medical Microbiology : IJMM·Jenny A Laverde GomezGuido Werner
Apr 10, 2013·The Open Microbiology Journal·Christina KourtesiGeorge P Tegos
Jul 12, 2013·The Journal of Antimicrobial Chemotherapy·Marius LinkeviciusDan I Andersson
Oct 29, 2014·Antimicrobial Agents and Chemotherapy·Vincent CattoirJean-Christophe Giard
Sep 15, 2015·International Journal of Antimicrobial Agents·Marc NiebelNicholas Loman

❮ Previous
Next ❯

Citations

Jun 16, 2016·Frontiers in Microbiology·Ana M Guzman PrietoRob J L Willems
Sep 14, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Sara Hernando-AmadoJosé L Martínez
Aug 16, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E-M KluppH Rohde
Oct 19, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Andrei Nicoli Gebieluca DabulIlana Lopes Baratella Cunha Camargo
Oct 24, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohamed O Ahmed, Keith E Baptiste
Jun 29, 2018·PloS One·Ahmed AbdelKhalekMohamed N Seleem
Jun 30, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Dorottya FranyóZsuzsanna Dombrádi
Mar 19, 2020·Molecular Microbiology·Fernando BaqueroTeresa M Coque
Jan 3, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Jennifer K BenderGuido Werner
Jun 25, 2017·The Journal of Antimicrobial Chemotherapy·Laura Di SanteFrancesca Biavasco
Mar 3, 2020·Frontiers in Microbiology·Leonardo G Panunzi
Aug 12, 2020·Antimicrobial Resistance and Infection Control·Xuewei ZhouErik Bathoorn
Apr 5, 2018·Pathogens and Disease·Gregory H TysonPatrick F McDermott
Feb 1, 2019·Clinical Microbiology Reviews·Mónica García-Solache, Louis B Rice
Jan 17, 2019·The Journal of Antimicrobial Chemotherapy·Gianluca MorroniFrancesca Biavasco
Oct 19, 2017·Bioscience of Microbiota, Food and Health·Yuji Ohashi, Tomohiko Fujisawa
Oct 14, 2020·Antimicrobial Agents and Chemotherapy·Hong YaoXiang-Dang Du
Dec 22, 2020·Frontiers in Microbiology·Nicolas HelsensCatherine Magras
Oct 31, 2020·International Journal of Antimicrobial Agents·Sarrah BoukthirVincent Cattoir
Jan 7, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Sajad YaghoubiFarajolah Maleki
Jan 27, 2021·Antimicrobial Agents and Chemotherapy·Nader S AbutalebMohamed N Seleem
Apr 26, 2021·The Journal of Antimicrobial Chemotherapy·Loren DejoiesVincent Cattoir
Jul 13, 2019·Microbiology Spectrum·Elizabeth FioreMichael S Gilmore
Jan 13, 2018·Microbiology Spectrum·Engeline van DuijkerenStefan Schwarz
Nov 6, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Andrei Nicoli Gebieluca DabulIlana Lopes Baratella da Cunha Camargo
Sep 25, 2021·Cancer Control : Journal of the Moffitt Cancer Center·Matthew SnyderAliyah Baluch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.