Tiger, Bengal and Domestic Cat Embryos Produced by Homospecific and Interspecific Zona-Free Nuclear Transfer

Reproduction in Domestic Animals = Zuchthygiene
L N MoroDaniel Felipe Salamone

Abstract

The aim of this study was to evaluate three different cloning strategies in the domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by interspecific cloning (iSCNT) using Bengal (a hybrid formed by the cross of Felis silvestris and Prionailurus bengalensis) and tiger (Panthera tigris) donor cells. In experiment 1, zona-free (ZP-free) cloning resulted in higher fusion and expanded blastocyst rates with respect to zona included cloning techniques that involved fusion or injection of the donor cell. In experiment 2, ZP-free iSCNT and embryo aggregation (2X) were assessed. Division velocity and blastocyst rates were increased by embryo aggregation in the three species. Despite fewer tiger embryos than Bengal and cat embryos reached the blastocyst stage, Tiger 2X group increased the percentage of blastocysts with respect to Tiger 1X group (3.2% vs 12.1%, respectively). Moreover, blastocyst cell number was almost duplicated in aggregated embryos with respect to non-aggregated ones within Bengal and tiger groups (278.3 ± 61.9 vs 516.8 ± 103.6 for Bengal 1X and Bengal 2X groups, respectively; 41 vs 220 ± 60 for Tiger 1X and Tiger 2X groups, respectively). OCT4 analysis also revealed that tiger b...Continue Reading

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Citations

Apr 14, 2016·In Vitro Cellular & Developmental Biology. Animal·Uma Mahesh YelisettiSambasiva Rao Brahmasani
Oct 2, 2019·Journal of Veterinary Research·Joanna KochanJózef Skotnicki
Oct 11, 2016·Current Protocols in Molecular Biology·Ana Cecilia Mestre-CitrinovitzPatricia Saragüeta
Nov 16, 2020·Reproduction in Domestic Animals = Zuchthygiene·Patrycja MrowiecWiesława Młodawska
Feb 28, 2020·Theriogenology·Ampika ThongphakdeeKaywalee Chatdarong

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