Tight Chemomechanical Coupling of the F1 Motor Relies on Structural Stability.

Biophysical Journal
Mana TanakaEiro Muneyuki

Abstract

The F1 motor is a rotating molecular motor that ensures a tight chemomechanical coupling between ATP hydrolysis/synthesis reactions and rotation steps. However, the mechanism underlying this tight coupling remains to be elucidated. In this study, we used electrorotation in single-molecule experiments using an F1βE190D mutant to demonstrate that the stall torque was significantly smaller than the wild-type F1, indicating a loose coupling of this mutant, despite showing similar stepping torque as the wild-type. Experiments on the ATPase activity after heat treatment and gel filtration of the α3β3-subcomplex revealed the unstable structure of the βE190D mutant. Our results suggest that the tight chemomechanical coupling of the F1 motor relies on the structural stability of F1. We also discuss the difference between the stepping torque and the stall torque.

References

Mar 20, 1997·Nature·H NojiK Kinosita
Mar 30, 2001·The Journal of Biological Chemistry·H NojiK Kinosita
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Hirono-HaraM Yoshida
Dec 11, 2002·Essays in Biochemistry·K KinositaH Noji
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Katsuya ShimabukuroMasasuke Yoshida
Jan 20, 2004·Nature Structural & Molecular Biology·Takayuki NishizakaKazuhiko Kinosita
Jan 30, 2004·Nature·Hiroyasu ItohKazuhiko Kinosita
Jan 1, 2005·Biophysical Journal·Naoyoshi SakakiKazuhiko Kinosita
Feb 18, 2005·Nature·Yannick RondelezHiroyuki Noji
Dec 13, 2006·Biophysical Journal·Eiro MuneyukiHiroyuki Noji
Mar 14, 2007·The Journal of Biological Chemistry·Matthew W BowlerJohn E Walker
Aug 28, 2007·Nature Structural & Molecular Biology·Takayuki ArigaMasasuke Yoshida
Dec 18, 2007·Biochemical and Biophysical Research Communications·Takahiro Watanabe-NakayamaEiro Muneyuki
Sep 28, 2010·Physical Review Letters·Shoichi ToyabeEiro Muneyuki
Sep 28, 2010·Physical Review Letters·Kumiko HayashiHiroyuki Noji
Oct 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shoichi ToyabeEiro Muneyuki
May 3, 2012·Journal of the American Chemical Society·Shigehiko HayashiHiroyuki Noji
Sep 27, 2012·Biophysical Journal·Mizue TanigawaraHiroyuki Noji
Feb 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·James L MartinWayne D Frasch
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ei-ichiro SaitaMasasuke Yoshida
Nov 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sándor Volkán-KacsóRudolph A Marcus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.