Tightly-bound ubiquinone in the Escherichia coli respiratory complex I

Biochimica Et Biophysica Acta
Michael I VerkhovskyMarina Verkhovskaya

Abstract

NADH:ubiquinone oxidoreductase (Complex I), the electron input enzyme in the respiratory chain of mitochondria and many bacteria, couples electron transport to proton translocation across the membrane. Complex I is a primary proton pump; although its proton translocation mechanism is yet to be known, it is considered radically different from any other mechanism known for redox-driven proton pumps: no redox centers have been found in its membrane domain where the proton translocation takes place. Here we studied the properties and the catalytic role of the enzyme-bound ubiquinone in the solubilized, purified Complex I from Escherichia coli. The ubiquinone content in the enzyme preparations was 1.3±0.1 per bound FMN residue. Rapid mixing of Complex I with NADH, traced optically, demonstrated that both reduction and re-oxidation kinetics of ubiquinone coincide with the respective kinetics of the majority of Fe-S clusters, indicating kinetic competence of the detected ubiquinone. Optical spectroelectrochemical redox titration of Complex I followed at 270-280nm, where the redox changes of ubiquinone contribute, did not reveal any transition within the redox potential range typical for the membrane pool, or loosely bound ubiquinone (...Continue Reading

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Citations

Feb 19, 2013·Nature·Rozbeh BaradaranLeonid A Sazanov
Jan 2, 2016·Biochimica Et Biophysica Acta·Judy Hirst, Maxie M Roessler
Sep 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Vivek SharmaVille R I Kaila
Nov 13, 2013·Biochimica Et Biophysica Acta·Marina Verkhovskaya, Mårten Wikström
Feb 20, 2015·Chemical Reviews·Mårten WikströmGerhard Hummer
Sep 18, 2012·The International Journal of Biochemistry & Cell Biology·Marina Verkhovskaya, Dmitry A Bloch
Dec 18, 2019·Science Advances·Kristian PareyVolker Zickermann
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Andrea Di LucaVille R I Kaila
Apr 27, 2019·Frontiers in Chemistry·Outi HaapanenVivek Sharma
Jan 14, 2021·International Journal of Molecular Sciences·Margherita Protasoni, Massimo Zeviani
Apr 17, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Outi Haapanen, Vivek Sharma
Feb 9, 2018·Biochimica Et Biophysica Acta. Bioenergetics·Andrea Di Luca, Ville R I Kaila
May 18, 2021·Frontiers in Chemistry·Etienne Galemou YogaVolker Zickermann
Oct 12, 2017·Journal of the American Chemical Society·Ana P Gamiz-HernandezVille R I Kaila

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