Tilmicosin and tylosin have anti-inflammatory properties via modulation of COX-2 and iNOS gene expression and production of cytokines in LPS-induced macrophages and monocytes

International Journal of Antimicrobial Agents
Xing-Yuan CaoBing-Yu Li

Abstract

Macrolides have been reported to modify the host immune and inflammatory responses both in vivo and in vitro. We examined the in vitro effect of the macrolides tilmicosin and tylosin, which are only used in the veterinary clinic, on the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)) and cytokines by lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and mouse peripheral blood mononuclear cells (PBMCs). Compared with 5 microg/mL, tilmicosin and tylosin concentrations of 10 microg/mL and 20 microg/mL significantly decreased the production of 6-keto-prostaglandin F(1alpha) (6-keto-PGF(1alpha)), PGE(2), NO, tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta and IL-6, and increased IL-10 production. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression were also significantly reduced. These results support the opinion that macrolides may exert an anti-inflammatory effect through modulating the synthesis of several mediators and cytokines involved in the inflammatory process.

Citations

Dec 18, 2013·Research in Veterinary Science·H WynsS Croubels
Nov 9, 2015·Veterinary Immunology and Immunopathology·Heidi WynsSiska Croubels
May 20, 2008·International Immunopharmacology·Xuemei ZhangXuming Deng
Jun 24, 2015·Veterinary Immunology and Immunopathology·H WynsS Croubels
Jun 11, 2017·Journal of Veterinary Pharmacology and Therapeutics·M Mohammadsadegh
Sep 17, 2017·The Veterinary Record·Alana RedfernAlbert Jergens
Jan 16, 2020·Animals : an Open Access Journal From MDPI·Carlo PinnaGiacomo Biagi
Mar 25, 2011·Immunopharmacology and Immunotoxicology·Xinxin CiXuming Deng
Nov 2, 2019·Journal of Veterinary Internal Medicine·Alison C ManchesterJan S Suchodolski

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