Tilted view reconstruction in optical microscopy. Three-dimensional reconstruction of Drosophila melanogaster embryo nuclei

Biophysical Journal
P J ShawJ W Sedat

Abstract

The resolution along the optical axis (z) is much less than the in-plane resolution in any current optical microscope, conventional or otherwise. We have used mutually tilted, through-focal section views of the same object to provide a solution to this problem. A tilting specimen stage was constructed for an optical microscope, which with the use of a coverslip-free water immersion lens, allowed the collection of data sets from intact Drosophila melanogaster embryos at viewing directions up to 90 degrees apart. We have devised an image processing scheme to determine the relative tilt, translation, and sampling parameters of the different data sets. This involves the use of a modified phase cross-correlation function, which produces a very sharp maximum. Finally the data sets are merged using figure-of-merit and local area scaling techniques borrowed from x-ray protein crystallography. We demonstrate the application of this technique to data sets from a metaphase plate in an embryo of Drosophila melanogaster. As expected, the merged reconstruction combined the highest resolution available in the individual data sets. As estimated from the Fourier transform, the final resolution is 0.25 microns in x and y and 0.4 microns in z. In...Continue Reading

Citations

Jan 1, 1991·Progress in Biophysics and Molecular Biology·P J Shaw, D J Rawlins
Dec 12, 2002·Micron : the International Research and Review Journal for Microscopy·M KozubekM Hausmann
Aug 2, 2003·Journal of Structural Biology·Hanspeter Winkler, Kenneth A Taylor
Feb 1, 1993·The Journal of Cell Biology·Y HiraokaJ W Sedat
Oct 1, 1995·The Journal of Cell Biology·Y UrataJ W Sedat
May 1, 1990·Journal of Microscopy·P J Shaw
May 26, 2009·Development·Jan Huisken, Didier Y R Stainier
Oct 6, 2007·Cell Structure and Function·Takashi HottaKoichi Mizuno
Jan 21, 2014·Protoplasma·Emilio GualdaGabriel G Martins
Mar 25, 2016·Micron : the International Research and Review Journal for Microscopy·T MuellerE Reithmeier
May 7, 2015·Journal of Microscopy·T BrunsH Schneckenburger
Oct 6, 1999·Current Opinion in Structural Biology·M G Gustafsson
Sep 13, 2013·Journal of Neuroscience Methods·Sadahiro IwabuchiN Charles Harata
Jul 15, 2004·PLoS Biology·Daniella Bianchi-FriasSusan M Parkhurst
Oct 27, 2004·Biophysical Journal·Peter M KassonAxel T Brunger
Jun 25, 2007·Optics Express·Jim SwogerErnst H K Stelzer
Sep 8, 2004·Microscopy Research and Technique·Michal KozubekStanislav Kozubek
Nov 30, 2007·Microscopy Research and Technique·Danai LaksameethanasanSpencer L Shorte
Nov 20, 2008·Journal of Microscopy·K T SealeJ P Wikswo
Feb 18, 2014·Nature Methods·Sharon Grayer WolfMichael Elbaum
Aug 3, 2002·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Rainer HeintzmannChristoph Cremer
Oct 15, 2008·Optics Express·Moritz K KreysingJosef A Käs
Feb 18, 2003·Journal of Microscopy·P MatulaM Hausmann
Sep 19, 2003·Optics Letters·Jim SwogerErnst H K Stelzer
Nov 13, 2015·Applied Optics·Micah H Jenkins, Thomas K Gaylord
Jun 8, 2012·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Vadim Y SolovievJorge Ripoll
Oct 18, 2011·Optics Letters·Guoan ZhengChanghuei Yang
Jul 30, 2014·Biomedical Optics Express·Yoshio Tanaka, Shin-Ich Wakida
May 2, 2019·Optics Letters·Esther H R TsaiManuel Guizar-Sicairos
Mar 7, 2020·Biomedical Optics Express·Gaszton VizsnyiczaiLóránd Kelemen
Jul 8, 2011·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Vadim Y Soloviev, Simon R Arridge
Mar 18, 2011·Biomedical Optics Express·Vadim Y Soloviev, Simon R Arridge
Dec 21, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Vadim Y SolovievSimon R Arridge

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