Time- and dose-dependent effects of protein kinase C on proximal bicarbonate transport

The Journal of Membrane Biology
T Wang, Y L Chan

Abstract

Activation of protein kinase C has been shown to cause both stimulation and inhibition of transport processes in the brush-border membrane and renal tubule. This study was designed to examine the dose-response nature and time-dependent effect of 4 beta-phorbol-12-myristate-13-acetate (PMA) on the rates of bicarbonate absorption (JHCO3) and fluid absorption (Jv) in the proximal convoluted tubule (PCT) of rat kidney. Bicarbonate flux was determined by total CO2 changes between the collected fluid and the original perfusate as analyzed by microcalorimetry. Luminal perfusion of PMA (10(-10) approximately 10(-5) M) within 10 min caused a significant increase of JHCO3 and Jv. A peaked curve of the dose response was observed with maximal effect at 10(-8) M PMA on both bicarbonate and fluid reabsorption, which could be blocked completely by amiloride (10(3) M) and EIPA (10(-5) M). On the other hand, with an increase of perfusion time beyond 15 min. PMA (10(-8) and 10(-6) M) could inhibit JHCO3 and Jv. Amiloride (10(-3) M) or EIPA (10(-5) M) significantly inhibits JHCO3 and Jv, while there is no additive effect of PMA and amiloride or EIPA on PCT transport. An inactive phorbol-ester, 4 alpha-phorbol, that does not activate protein kinas...Continue Reading

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Citations

May 9, 2006·Pflügers Archiv : European journal of physiology·Luciene Regina Carraro-Lacroix, Gerhard Malnic
Sep 1, 1992·Acta Physiologica Scandinavica·C F MendezA Aperia
Sep 15, 2005·American Journal of Physiology. Renal Physiology·Clint PerryIrina I Grichtchenko
Oct 27, 1999·Canadian Journal of Physiology and Pharmacology·F GagnonS N Orlov
May 18, 1999·The American Journal of Physiology·M R WiederkehrO W Moe
Jun 3, 2004·American Journal of Physiology. Renal Physiology·Matthew A Bailey
Jun 5, 1998·The American Journal of Physiology·T J ThekkumkaraS L Linas
Jan 16, 2003·American Journal of Physiology. Renal Physiology·Zhaopeng DuTong Wang
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Tong Wang
Jan 14, 2005·The Journal of Biological Chemistry·L'Aurelle A JohnsonMargaret E Gnegy
May 1, 1995·The American Journal of Physiology·E FérailleH Favre

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