Time- and dose-related effects of amyloid beta1-40 on retina and optic nerve morphology in rats

The International Journal of Neuroscience
Mohd Aizuddin Mohd LazaldinNafeeza Mohd Ismail

Abstract

Amyloid beta (Aβ) is known to contribute to the pathophysiology of retinal neurodegenerative diseases such as glaucoma. Effects of intravitreal Aβ(1-42) on retinal and optic nerve morphology in animal models have widely been studied but not those of Aβ(1-40). Hence, we evaluated the time- and dose-related effects of intravitreal Aβ(1-40) on retinal and optic nerve morphology. Since oxidative stress and brain derived neurotrophic factor (BDNF) are associated with Aβ-induced neuronal damage, we also studied dose and time-related effects of Aβ(1-40) on retinal oxidative stress and BDNF levels. Five groups of rats were intravitreally administered with vehicle or Aβ(1-40) in doses of 1.0, 2.5, 5 and 10 nmol. Animals were sacrificed and eyes were enucleated at weeks 1, 2 and 4 post-injection. The retinae were subjected to morphometric analysis and TUNEL staining. Optic nerve sections were stained with toluidine blue and were graded for neurodegenerative effects. The estimation of BDNF and markers of oxidative stress in retina were done using ELISA technique. It was observed that intravitreal Aβ(1-40) causes significant retinal and optic nerve damage up to day 14 post-injection and there was increasing damage with increase in dose. Ho...Continue Reading

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Citations

Sep 21, 2018·Neural Regeneration Research·Natasha Najwa Nor ArfuzirNafeeza Mohd Ismail
Dec 29, 2019·The European Journal of Neuroscience·Mohd Aizuddin Mohd LazaldinNafeeza Mohd Ismail
Mar 28, 2020·Science Translational Medicine·Xiaowei WangMaiken Nedergaard
Mar 4, 2021·International Journal of Molecular Sciences·Liang Wang, Xiaobo Mao

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