Time and concentration dependence of Fenton-induced oxidation of dG

Nucleosides, Nucleotides & Nucleic Acids
Carl Elovson Grey, Patrick Adlercreutz

Abstract

The influence of incubation time and Fenton reagent concentrations was investigated on the oxidation of 2'-deoxyguanosine. The compounds identified and quantified, through use of an LC-MS/MS system, were 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8,5'-cyclo-2'-deoxyguanosine (8,5'cyclodG) and the secondary oxidation products guanidinohydantoin and dehydro-guanidinohydantoin. 8-oxodG and 8,5' cyclodG formed very quickly, reaching a maximum rapidly, but with 8-oxodG a rapid decline occurred thereafter due to its further oxidation into the secondary products, which formed more slowly. Due to the better stability, 8,5' cyclodG correlated better with the general level of oxidation than 8-oxodG. The results emphasize the advantages of measuring other oxidation adducts than 8-oxodG alone.

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Citations

May 12, 2007·Chembiochem : a European Journal of Chemical Biology·Carl Elovson GreyPatrick Adlercreutz
Nov 26, 2009·Chemistry : a European Journal·Tuomas LönnbergMakoto Komiyama

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Methods Mentioned

BETA
NMR
MDS

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