Time, cost, and efficacy study of identifying group A streptococci with commercially available reagents.

Applied Microbiology
L A ChitwoodH D Riley

Abstract

During the 12-month period primary throat, wound, and skin cultures, tentatively identified as B streptococci, were submitted by 10 different clinical laboratories for evaluation. A total of 692 beta-hemolytic streptococci were isolated from cultures submitted and examined in parallel by the fluorescent-antibody, precipitin, and bacitracin techniques. An evaluation of the specificity and sensitivity in conjunction with basic and personnel costs was determined for each method. The standard Lancefield precipitin method was established as the standard by which the bacitracin and flourescent antibody techniques were compared. With some variation depending on the commerical source of the disc, approximately 7% of the strains examined produced false reactions with the bacitracin disc. False-negative reactions were rarely noted by the group A fluorescent antibody technique (0.5%), but an appreciable number of other Lancefield groups (B, C, and G) were nonreactive with homologous conjugates.

References

Oct 1, 1965·American Journal of Public Health and the Nation's Health·A A GroverA S Evans
Aug 1, 1953·Journal of Clinical Pathology·W R MAXTED
Aug 1, 1957·American Journal of Public Health and the Nation's Health·R W QUINNH D RILEY
Jul 1, 1961·Journal of Bacteriology·J O COHENW B CHERRY
Nov 23, 1964·JAMA : the Journal of the American Medical Association·A S EVANS, E C DICK
Mar 1, 1955·Journal of Bacteriology·M L LEVINSON, P F FRANK
May 13, 1950·Journal of the American Medical Association·F W DENNYE A CUSTER
Jul 1, 1963·American Journal of Public Health and the Nation's Health·M D MoodyC C Winter
Dec 4, 1948·Journal of the American Medical Association·B F MASSELLT D JONES

❮ Previous
Next ❯

Citations

Dec 1, 1973·Applied Microbiology·E A Edwards, G L Larson
Jan 1, 1980·Journal of Clinical Microbiology·H D RoseG Witte
Oct 1, 1980·Journal of Clinical Microbiology·M H NahmJ M Davie
Mar 11, 1975·Journal of Clinical Microbiology·B K WatsonR C Moellering
May 1, 1978·Journal of Clinical Microbiology·R A Stoner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.