Time-course and treatment response with SNX-111, an N-type calcium channel blocker, in a rodent model of focal cerebral ischemia using diffusion-weighted MRI

Brain Research
M A YenariG K Steinberg

Abstract

Diffusion-weighted magnetic resonance imaging (DWI) is capable of noninvasively imaging acute cerebral ischemia. We demonstrate the utility of this technique by evaluating SNX-111, a novel N-type calcium channel blocker with potential neuroprotective properties, in a rodent model of transient focal ischemia. Twenty-four Sprague-Dawley rats weighing between 310-350 g underwent occlusion of the middle cerebral artery (MCAO) for 105 min followed by 22.5 h of reperfusion. Thirty minutes following MCAO, animals were randomized to receive SNX-111 5 mg/kg intravenously over 1 h vs. placebo. DWI and T2-weighted MRIs (T2W) were performed at 0.5, 1.5 and 24 h after the onset of ischemia. Area fractions of increased signal intensity on the DWI and T2W images were measured. DWI area fractions at 1.5 and 24 h were also normalized to the initial, pre-treatment scans. Apparent diffusion coefficients (ADC) were calculated from fitted maps. Tri-phenyl tetrazolium chloride (TTC) staining was performed on brains at 24 h and infarct area fractions were measured. SNX-111 treated animals showed significantly improved 1.5-h DWI scan ratios compared to controls (ratios of 1.06 +/- 0.25 vs. 2.98 +/- 0.78 SNX vs. controls respectively, P < 0.05). A tren...Continue Reading

References

Sep 1, 1992·Stroke; a Journal of Cerebral Circulation·K MinematsuM Fisher
Jul 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H ChenJ H Garcia
Jan 1, 1990·Journal of Cardiovascular Pharmacology·A Sauter, M Rudin
Mar 1, 1991·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J MintorovitchP R Weinstein
Jul 20, 1990·Science·B M OliveraL J Cruz
Nov 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M JacewiczW A Pulsinelli
May 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M E MoseleyP R Weinstein
Jan 2, 1987·Science·R J Miller
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E W McCleskeyD Yoshikami
Oct 1, 1988·Trends in Neurosciences·R W TsienA P Fox
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Jan 28, 1988·The New England Journal of Medicine·H J GelmersH J Wiezer
Apr 1, 1988·Stroke; a Journal of Cerebral Circulation·J C GrottaD McCandless
Sep 1, 1988·Stroke; a Journal of Cerebral Circulation·G K SteinbergT Gross
Dec 20, 1985·Science·B M OliveraL J Cruz
Jan 1, 1984·Acta Neuropathologica·T M LiszczakN T Zervas
Aug 1, 1995·Stroke; a Journal of Cerebral Circulation·T B MüllerA N Oksendal
Jul 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D SauerP R Allegrini
Nov 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A M BuchanH J Lesiuk
Jul 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E H LoD Luttinger
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K ValentinoR Ciaranello
Feb 1, 1994·Stroke; a Journal of Cerebral Circulation·T BackK A Hossmann
Sep 1, 1993·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·B J DardzinskiK Minematsu

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Citations

Oct 20, 1998·Annals of Neurology·M A YenariG K Steinberg
Feb 16, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Francis G BlankenbergMidori Anne Yenari
May 19, 2009·Neuroscience Bulletin·Zhao-Hui GuoWei-Zhi Wang
Nov 25, 2000·European Journal of Pharmacology·C A HicksM J O'Neill
Jan 4, 2001·European Journal of Pharmacology·L BogaertY Michotte
Jun 16, 2012·International Journal of Nanomedicine·Sung Ung MoonJong Eun Lee
May 27, 2005·Neurosurgical Focus·M Ross BullockGraham M Teasdale
Jun 17, 2014·Zeitschrift für medizinische Physik·Frank G ZöllnerLothar R Schad
Jun 28, 2011·European Journal of Pediatrics·Arvind Sehgal
Dec 16, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Joshua Y ChuaRaphael Guzman
May 9, 2002·Expert Opinion on Investigational Drugs·Jeffrey J LegosFrank C Barone
Jun 2, 2005·Expert Opinion on Investigational Drugs·Valentin K Gribkoff, Raymond J Winquist
Sep 10, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Shohei MatsumotoTadeusz Wieloch
Apr 3, 2001·Stroke; a Journal of Cerebral Circulation·M A YenariG K Steinberg
Feb 11, 2015·Translational Neuroscience·Peng-Fei WangQing-Wu Yang
Oct 31, 1997·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·M A YenariM E Moseley
May 30, 2017·Neural Regeneration Research·Hai-Feng MaoChu-Zu Wu
Nov 13, 2003·Expert Opinion on Emerging Drugs·Isabel J BeresfordA Jacqueline Hunter

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