Time-course effects of increased fatty acid supply on the expression of genes involved in lipid/glucose metabolism in muscle cells

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Ana M RodríguezAndreu Palou

Abstract

Fatty acid (FA) oversupply in skeletal muscle is related with metabolic disorders associated to obesity, and also with normal physiological responses. We studied, in vivo and in vitro, the chronological response to physiological increases of FA, analyzing the expression of selected genes important for glucose/lipid metabolism. An in vivo sequential model of fasting (known to increase circulating FA) and refeeding was used in male Wistar rats to study soleus (more oxidative) and gastrocnemius (more glycolytic) muscles, and a chronological study was made in C2C12 muscle cells under treatment of oleic/linoleic FA mixture, at physiological concentration. Body weight, muscle glycogen and blood parameters (glucose, insulin, free fatty acids -FFA-, triglycerides) were monitored. mRNA levels of muscle carnitine palmitoyl transferase 1 (mCPT1), GLUT 4, insulin receptor (InsR), MyoD1, peroxisome proliferator activated receptor (PPAR) gamma coactivator 1alpha (PGC1alpha) and beta (PGC1beta), PPARalpha, PPARdelta, pyruvate dehydrogenase kinase 4 (PDK4) and uncoupling proteins (UCPs) 2 and 3 were analyzed by quantitative RT-PCR. The main results were the quick induction of PGC1alpha, UCP3 and PDK4 in vivo (more marked in gastrocnemius) and ...Continue Reading

Citations

Jun 12, 2012·Nutrition & Metabolism·Rita Rinnankoski-TuikkaHeikki Kainulainen
Dec 26, 2013·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·A A TurcoB Capaldo
Oct 17, 2012·Molecular and Cellular Endocrinology·L M CornallA J McAinch
Jul 2, 2011·Experimental Eye Research·Juan C ArciniegaIgor A Butovich
May 8, 2013·Molecular Nutrition & Food Research·Juana SánchezAna M Rodríguez
Sep 16, 2011·American Journal of Physiology. Endocrinology and Metabolism·Angélica Ruiz-RamírezMohammed El-Hafidi

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