Time-Course Microarray Analysis Reveals Differences between Transcriptional Changes in Tomato Leaves Triggered by Mild and Severe Variants of Potato Spindle Tuber Viroid

Viruses
Aneta WięsykAnna Góra-Sochacka

Abstract

Viroids are small non-capsidated non-coding RNA replicons that utilize host factors for efficient propagation and spread through the entire plant. They can incite specific disease symptoms in susceptible plants. To better understand viroid-plant interactions, we employed microarray analysis to observe the changes of gene expression in "Rutgers" tomato leaves in response to the mild (M) and severe (S23) variants of potato spindle tuber viroid (PSTVd). The changes were analyzed over a time course of viroid infection development: (i) the pre-symptomatic stage; (ii) early symptoms; (iii) full spectrum of symptoms and (iv) the so-called 'recovery' stage, when stem regrowth was observed in severely affected plants. Gene expression profiles differed depending on stage of infection and variant. In S23-infected plants, the expression of over 3000 genes was affected, while M-infected plants showed 3-fold fewer differentially expressed genes, only 20% of which were specific to the M variant. The differentially expressed genes included many genes related to stress; defense; hormone metabolism and signaling; photosynthesis and chloroplasts; cell wall; RNA regulation, processing and binding; protein metabolism and modification and others. Th...Continue Reading

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Citations

Aug 6, 2020·International Journal of Molecular Sciences·Charith Raj Adkar-Purushothama, Jean-Pierre Perreault
Aug 13, 2021·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Teruo Sano

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Datasets Mentioned

BETA
X76846
X76844
GSE106912

Methods Mentioned

BETA
dot-blot
PCR
RNA-seq

Software Mentioned

MapMan
Affymetrix
Lasergene
STATISTICA
Venny
Partek Genomics Suite
nCounter CodeSet
nCounter
Blast2GO

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