PMID: 9174648May 1, 1997Paper

Time course of calcium transients derived from Fura-2 fluorescence measurements in single fast twitch fibres of adult mice and rat myotubes developing in primary culture

Cell Calcium
A J BakkerD G Stephenson

Abstract

In this study, we applied a method to correct for the altered binding kinetics of Fura-2 for Ca2+ in vivo, on Ca2+ fluorescence transients (Ca2+F) measured using Fura-2 in single adult fast twitch skeletal muscle fibres of the mouse, which exhibit very fast [Ca2+] responses, and rat myotubes developing in culture which exhibit slower [Ca2+] responses (rise time [20-80% of peak] of Ca2+F transients: 1.81 +/- 0.17 ms and 16.14 +/- 2.60 ms, respectively). After correction, the [Ca2+] transients (Ca2+C) measured in both the adult mouse fibres and the myotubes rose more rapidly (mean rise time of Ca2+C transients: adult mouse fibres, 0.76 +/- 0.12 ms; rat myotubes, 8.25 +/- 2.83 ms) and often exhibited a Ca2+ spike which exceeded the peak of the Ca2+F transient. In the adult mouse fibres, correction increased the mean peak [Ca2+] of the Ca2+F transients by a factor of 7 from 0.53 +/- 0.08 microM to 3.76 +/- 0.71 microM. The accuracy of the time course of the corrected Ca2+ transients was confirmed by comparison to the time course of Ca2+ transients measured with Mag-Fura-5, which had a similar mean rise time (0.94 +/- 0.10 ms, t-test, P = 0.80). The more slowly rising Ca2+ transients measured in the rat myotubes were less affected b...Continue Reading

References

Jan 1, 1991·The Japanese Journal of Physiology·N Suda, S Kurihara
Sep 1, 1990·The Journal of Physiology·D A WilliamsD G Stephenson
Sep 1, 1988·The Journal of Physiology·S M Baylor, S Hollingworth
Jul 1, 1995·The American Journal of Physiology·S L CarrollM F Schneider
Apr 1, 1993·The Journal of Physiology·O Delbono, E Stefani
Jan 1, 1993·The Journal of Physiology·A J BakkerD G Stephenson
Nov 1, 1996·The Journal of General Physiology·S HollingworthS M Baylor

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Citations

Dec 17, 2003·The Journal of General Physiology·R P Schuhmeier, W Melzer
Sep 9, 1999·In Vitro Cellular & Developmental Biology. Animal·G L PortiérT H van Kuppevelt
Jul 27, 2007·Cell Motility and the Cytoskeleton·Gianina RavenscroftNigel G Laing

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