Time course of changes in in vitro sarcoplasmic reticulum Ca2+-handling and Na+-K+-ATPase activity during repetitive contractions

Pflügers Archiv : European journal of physiology
Takaaki MishimaMasanobu Wada

Abstract

Time-dependent changes in sarcoplasmic reticulum (SR) Ca2+-handling and Na+-K+-ATPase activity, as assessed in vitro, were investigated in the superficial (GS) and deep regions (GD) of rat gastrocnemius muscles undergoing short-term (up to 30 min) electrical stimulation. There was a rapid and progressive loss of force output during the first 5 min of stimulation. For GS, significant depressions (P < 0.05) in SR Ca2+-uptake rate and Ca2+-ATPase activity were observed during only the first 1 min. No further reductions occurred with stimulation time. SR Ca2+-release rate was significantly (P < 0.05) decreased at 3 min. For GD, significant reductions (P < 0.05) in Ca2+-uptake rate, Ca2+-release rate and Ca2+-ATPase activity were manifested after 3, 5, and 5 min, respectively. A decay in Na+-K+-ATPase activity was found only in 1-min stimulated GD and 30-min stimulated GS. After 30 min, the depressed functions reverted to resting levels in GD but not in GS. The alterations in any variables examined were not parallel with changes in force output. These results suggest that, at least under the conditions used in this study, in vivo disruptions in cation regulation mediated by vigorous contractile activity would be attributable primari...Continue Reading

References

Dec 1, 1990·Analytical Biochemistry·W S Simonides, C van Hardeveld
May 2, 1998·Analytical Biochemistry·S F Fraser, M J McKenna
Mar 30, 2001·The Journal of Biological Chemistry·J SunG Meissner
Sep 12, 2002·Journal of Muscle Research and Cell Motility·S MatsunagaD Pette
Apr 10, 2003·Pflügers Archiv : European journal of physiology·Satoshi MatsunagaMasanobu Wada
Jun 26, 2003·The Journal of Physiology·Pasi TaviHåkan Westerblad
Apr 22, 2004·Advances in Experimental Medicine and Biology·Håkan Westerblad, David G Allen
Jun 17, 2004·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·Howard J Green
Jun 17, 2004·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·A Russell Tupling
Jun 17, 2004·Canadian Journal of Applied Physiology = Revue Canadienne De Physiologie Appliquée·Rene Vandenboom
Aug 10, 2004·Clinical and Experimental Pharmacology & Physiology·D G Allen
Aug 25, 2004·Science·David Allen, Håkan Westerblad
Aug 20, 2005·Journal of Applied Physiology·S D E SandifordJ Ouyang
Oct 18, 2006·Clinical and Experimental Pharmacology & Physiology·D R Laver
Jan 18, 2007·European Journal of Applied Physiology·Satoshi MatsunagaMasanobu Wada

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Citations

Nov 3, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Daiki Watanabe, Masanobu Wada
Nov 7, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Carsten Juel
Jun 27, 2019·Journal of Muscle Research and Cell Motility·Daiki Watanabe, Masanobu Wada

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