Time course of enzyme changes after a switch from a high-fat to a low-fat diet

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
S P Brooks, B J Lampi

Abstract

This study was conducted to determine the time course of metabolic changes associated with a switch from a high-fat to a low-fat diet in rats. Adult rats, maintained on a high-fat diet (42% of energy from fat) for 4-5 weeks were switched to a low-fat diet (11% of energy from fat), and the activities of several liver enzymes were followed. Three different phases could be distinguished. The early phase, complete by 2 days after the switch in diets, included an increase in the activity of glucose 6-phosphate dehydrogenase (pentose phosphate pathway), an increase in pyruvate kinase and pyruvate dehydrogenase activities (terminal end of the glycolytic pathway) and an increase in ATP-citrate lyase and fatty acid synthetase (fatty acid synthesis pathway). The early phase also included a decrease in the activity of phosphoenolpyruvate carboxykinase (PEPCK, gluconeogenesis) and a lower branched-chain amino acid dehydrogenase activity (BCAADH, branched-chain amino acid degradation). The concentration of the allosteric phosphofructokinase regulator, fructose 2,6-bisphosphate (Fru-2,6-P2, glycolysis), decreased during the early phase. An intermediate phase could also be discerned between 3 and 10 days after the switch in diets. In this pha...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C KhooD Steinberg
Jan 1, 1975·Current Topics in Cellular Regulation·R Frenkel
Jan 1, 1978·The International Journal of Biochemistry·E R Hall, G L Cottam
Nov 15, 1992·European Journal of Biochemistry·T Nouspikel, P B Iynedjian
Jan 1, 1992·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Y CherelY Le Maho
Jun 1, 1991·The American Journal of Clinical Nutrition·M Y JacksonS Pelican
Jan 1, 1988·Annual Review of Biochemistry·S J PilkisT H Claus
Jan 1, 1986·Current Topics in Cellular Regulation·D K Srivastava, S A Bernhard
Jan 1, 1973·Annual Review of Biochemistry·J J Volpe, P R Vagelos
Aug 1, 1973·The American Journal of Clinical Nutrition·E Sohar, E Sneh
Oct 1, 1965·Journal of Cellular Physiology·H A LardyP D Ray
Jan 1, 1983·Annual Review of Biochemistry·S J WakilV C Joshi
Jun 1, 1983·Biochemical Society Transactions·L Hue
Sep 1, 1983·Archives of Biochemistry and Biophysics·O K ChaekalR A Harris
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J J AragónA Sols
Mar 20, 1995·Analytical Biochemistry·S P BrooksH G Botting
Jan 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Vaulont, A Kahn
Jun 14, 1993·Annals of the New York Academy of Sciences·J P Flatt
Jun 7, 1996·Molecular and Cellular Biochemistry·S P Brooks, B J Lampi
Jul 1, 1958·The Journal of Pediatrics·R S ILLINGWORTH
Jan 1, 1965·The Biochemical Journal·H A KREBS, L V EGGLESTON

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Citations

Aug 4, 2009·Cellular and Molecular Neurobiology·George BoukouvalasEfthimia Kitraki
Jun 12, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ma Enriqueta Muñoz, Elizabeth Ponce
Jul 26, 2012·American Journal of Physiology. Endocrinology and Metabolism·Peter A HeckerWilliam C Stanley
Feb 8, 2008·Nutrition & Metabolism·Richard L SeipGualberto Ruaño
May 3, 2001·The American Journal of Clinical Nutrition·B Mittendorfer, L S Sidossis
Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M E BizeauM J Pagliassotti

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