Time course of protein changes following in vitro ischemia in the rat hippocampal slice

Brain Research
K M Raley-Susman, J Murata

Abstract

Following 5 min in vitro ischemia, total protein synthesis is dramatically and persistently inhibited in neurons in the rat hippocampal slice. This model system was used to explore the responses of individual proteins to this irreversible insult. In vitro ischemia inhibited new protein synthesis of most proteins analyzed; however, the synthesis of a 68/70 kDa protein was substantially stimulated for the first hour after ischemia. By 3 hr postischemia, its synthesis rates were depressed to 60% of control rates. Although the total amounts of most proteins were not significantly depleted for the first few hours after ail ischemic episode, there were several notable exceptions. The levels of HSC73, a constitutively expressed member of the 70 kDa stress protein family, were reduced after in vitro ischemia. In addition, MAP-2 (microtubule-associated protein-2) and alpha-tubulin were depleted in the early hours after the insult, with MAP-2 exhibiting a detectable depletion earlier than tubulin. In contrast, the levels and distribution of a 68 kDa neurofilament protein localized to CA3 pyramidal neurons in the slice, apparently distinct from the band whose new synthesis was stimulated, were not affected by the 5 min in vitro ischemia i...Continue Reading

References

May 1, 1977·Journal of Neurochemistry·H K CooperP Kleihues
Jul 1, 1992·Brain Pathology·B E Dwyer, R N Nishimura
Aug 1, 1992·Annals of Medicine·M Jäättelä, D Wissing
Sep 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J KawagoeK Kogure
May 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R WidmannK A Hossmann
Jan 1, 1990·Acta Neuropathologica·T YanagiharaW E Mushynski
Feb 1, 1985·Journal of Neurochemistry·G A DienelS J Rosenfeld
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·T KuwakiT Nishimura
Feb 1, 1986·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W BodschK A Hossmann
Dec 24, 1971·Brain Research·P Kleihues, K A Hossmann
Jan 1, 1992·The European Journal of Neuroscience·S. PapasY. Ben-Ari
Jan 1, 1992·The European Journal of Neuroscience·C. Charriaut-MarlangueY. Ben-Ari

❮ Previous
Next ❯

Citations

May 26, 1999·Pharmacology & Therapeutics·K Nieber
Sep 11, 2012·Neuromolecular Medicine·Raquel HernándezMaría Á Peinado
Oct 11, 2003·Journal of Neurochemistry·Shane E MunnsPeter G Arthur
Dec 14, 2018·Oxidative Medicine and Cellular Longevity·Lingling Zhang, Jianzong Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.