PMID: 8596723Mar 1, 1996Paper

Time course of release of content of single vesicles in bovine chromaffin cells

Pflügers Archiv : European journal of physiology
A WalkerJ M Trifaró

Abstract

The time course of the spontaneous current spikes produced by release of the catecholamine contents of individual vesicles was examined in bovine chromaffin cells using carbon filament electrodes. The rate of spontaneous release was enhanced by adding either LaCl3 (0.01-0.5 mM) or BaCl2 (2 mM) to the extracellular solution. A paucity of events of very short duration was evident from the frequency histograms of the rise and the decay times. In the scatterograms of the rise and of the decay times the regression lines are invariably positive (i.e. the longer the duration of the rise times the longer the duration of the decay times). However, the regression lines never go through the origin but intercept the ordinate (the axis of the decay times) at (+/- SD) 16.1 +/- 6.4 ms (n = 11). On the other hand, the regression lines of paired rise and decay times for the time courses of diffusion are both linear and go through the origin. This relationship holds irrespective of whether the diffusion from an instantaneous point source was assumed to occur in an infinite plane or in an infinite volume. Therefore our experimental findings are incompatible with the model(s) assuming that diffusional broadening determines entirely the time course...Continue Reading

References

Feb 13, 1992·European Journal of Pharmacology·J M TrifaróA Rodríguez Del Castillo
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Citations

Mar 22, 2006·Biological cybernetics·H R RabieM I Glavinović
Sep 1, 1996·Pflügers Archiv : European journal of physiology·A WalkerJ Trifaró
Jun 25, 1998·Neuroscience·M I GlavinovićJ M Trifaró
Feb 9, 2005·Journal of Neuroscience Methods·S KebirM I Glavinović
Oct 4, 2006·Biochimica Et Biophysica Acta·G De Luca, M I Glavinović
Feb 12, 1998·The Journal of Biological Chemistry·C A AspinwallJ R Lakey
May 30, 2002·The Journal of Biological Chemistry·Kevin P Troyer, R Mark Wightman
Apr 5, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zohreh Chiti, Anja G Teschemacher
Mar 28, 1997·The Journal of Biological Chemistry·R BorgesR M Wightman

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