Time course proteomic profile of rat acute myocardial infarction by SELDI-TOF MS analysis

International Journal of Cardiology
Gengqian ZhangLin Zhang

Abstract

Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF MS) is a powerful tool to detect biomarkers of many diseases. The purpose of this study is to evaluate SELDI-TOF MS as a potential tool for identifying serological biomarkers of acute myocardial infarction (AMI) at an early stage. Serum samples were collected from rats after they had received left anterior descending coronary artery (LAD) ligation at 5, 15, 30, 60, 120, 240 and 360 min, respectively. These samples, along with serum samples from the sham-operated and uninjured control animals, were processed and analyzed with CM-10 ProteinChip and SELDI-TOF MS. Levels of myocardial creatine kinase isoenzyme MB (CKMB) activity and cardiac troponin I (cTnI) concentration were measured with respective commercial kits. Histological changes were examined after HE staining. Three peaks with m/z 7586 Da, 7564 Da and 9583 Da, which were only found in the operated groups, were termed specific peaks. The peaks with m/z 7586 and 7564 Da had the sensitivity of 97.0% and specificity of 97.0% in the >or=15 min operated groups. Four peaks with m/z 4983, 5140, 8075, and 9423 Da were novel tissue unspecific injury related peaks in response to injury. Four pe...Continue Reading

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Citations

Feb 26, 2013·Journal of Proteomics·Ivano EberiniElisabetta Gianazza
Mar 2, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Vivian Nogueira SilbigerMario Hiroyuki Hirata
Mar 29, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Vivian N SilbigerMario H Hirata
Mar 26, 2013·Molecular Medicine Reports·Yun LiuLin Zhang

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