Time-course transcriptional profiling of human amniotic fluid-derived stem cells using microarray.

Cancer Research and Treatment : Official Journal of Korean Cancer Association
Yong Wook KimWoong Shick Ahn

Abstract

To maintain the homeostasis of stem cells and prevent their ability to initiate tumorigenesis, it is important to identify and modify factors that prevent or accelerate stem cell senescence. We used microarrays to attempt to identify such factors in human amniotic fluid (HAF)-derived stem cells. To identify gene expression changes over a time course, we compared gene expression profiles of HAF-derived stem cells in different passages (1(st), 2(nd), 4(th), 6(th), 8(th), and 10(th)) using a Sentrix Human illumina microarray. Of the 25,804 genes in the microarray chip, 1,970 showed an over 2-fold change relative to the control (the 1(st) passage)-either upregulated or downregulated. Quantitative real-time PCR validated the microarray data for selected genes: markedly increased genes were CXCL12, cadherin 6 (CDH6), and folate receptor 3 (FOLR3). Downregulated genes included cyclin D2, keratin 8, insulin-like growth factor 2 (IGF2), natriuretic peptide precursor B (NPPB) and cellular retinoic acid binding protein 2 (CRABP2). The expression pattern of the selected genes was consistent with the microarray data except for CXCL12 and IGF2. Interestingly, the expression of NPPB was dramatically downregulated along the time course; it was...Continue Reading

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Citations

Oct 25, 2012·Stem Cells International·Kerry RennieMahmud Bani-Yaghoub
Apr 16, 2015·PloS One·Dalila Lucíola ZanetteWilson Araújo Silva
Jun 16, 2012·Stem Cells International·Maria G RoubelakisNicholas P Anagnou
Dec 7, 2018·Frontiers in Genetics·Yaroslav R EfremovSergey S Bogachev

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