Time-Course Transcriptome Analysis of Compatible and Incompatible Pollen-Stigma Interactions in Brassica napus L

Frontiers in Plant Science
Tong ZhangTingdong Fu

Abstract

Brassica species exhibit both compatible and incompatible pollen-stigma interactions, however, the underlying molecular mechanisms remain largely unknown. Here, RNA-seq technology was applied in a comprehensive time-course experiment (2, 5, 10, 20, and 30 min) to explore gene expression during compatible/incompatible pollen-stigma interactions in stigma. Moderate changes of gene expression were observed both in compatible pollination (PC) and incompatible pollination (PI) within 10 min, whereas drastic changes showed up by 30 min, especially in PI. Stage specific DEGs [Differentially Expressed Gene(s)] were identified, and signaling pathways such as stress response, defense response, cell wall modification and others were found to be over-represented. In addition, enriched genes in all samples were analyzed as well, 293 most highly expressed genes were identified and annotated. Gene Ontology and metabolic pathway analysis revealed 10 most highly expressed genes and 37 activated metabolic pathways. According to the data, downstream components were activated in signaling pathways of both compatible and incompatible responses, and incompatible response had more complicated signal transduction networks. This study provides more det...Continue Reading

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Citations

Feb 6, 2020·Journal of Integrative Plant Biology·Zhiqiang DuanChaozhi Ma
Apr 9, 2020·Acta Biochimica Et Biophysica Sinica·Zhiqiang DuanChaozhi Ma
Jun 6, 2018·International Journal of Molecular Sciences·Yong YangChaozhi Ma
Mar 25, 2021·Scientific Reports·Juan LobatonRomina Rader
Apr 4, 2021·Plants·Tassilo Erik WollenweberChristian Schulze Gronover
Apr 16, 2021·BMC Genomics·Chie KoderaIsabelle Fobis-Loisy

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Methods Mentioned

BETA
ubiquitination
transgenic
RNA-seq

Software Mentioned

Cufflinks
Blast2Go
InterProScan5
BlastP
TopHat
GO

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