PMID: 7514279Jan 1, 1994Paper

Time-dependent changes in central nicotinic acetylcholine channel kinetics in excised patches

Neuropharmacology
R A Lester, J A Dani

Abstract

The behavior of nicotinic acetylcholine receptor (nAChR) channels in acutely isolated habenula neurons was examined by rapidly applying nicotinic agonists to outside-out membrane patches. At negative membrane potentials, applications of 100 microM nicotine routinely produced macroscopic currents due to the opening of a large number of channels. During the continuous application of the agonist, the number of open nAChR channels decreased exponentially, i.e. receptor desensitization. A progressive loss in the number of channels contributing to the peak current was observed with time following outside-out patch excision, i.e. receptor rundown. In addition to rundown there was a time-dependent increase in the rate of desensitization and a concomitant slowing in the rate of recovery from desensitization. The extent of rundown and the changes in desensitization were coupled to the time after patch excision and were not dependent on ligand activation of nicotinic channels.

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Citations

Jun 6, 2000·Pharmacology, Biochemistry, and Behavior·G Tanda, S R Goldberg
Feb 14, 2002·Pharmacology, Biochemistry, and Behavior·J A Dani, M De Biasi
Mar 1, 1996·Trends in Neurosciences·M V Jones, G L Westbrook
Oct 1, 1995·Journal of Neuroendocrinology·D T Coleman, C Bancroft
Feb 1, 1995·British Journal of Pharmacology·J M Nooney, A Feltz
Jul 22, 1999·Annals of the New York Academy of Sciences·C P FensterR A Lester
May 1, 1996·Neuron·J A Dani, S Heinemann
Oct 24, 2015·Cerebral Cortex·Marcelo Di Marcello Valladão LugonMichael A Nitsche
Dec 6, 1997·Nature·V I PidoplichkoJ A Dani
Nov 19, 2002·Journal of Neurobiology·Michael W Quick, Robin A J Lester
Oct 20, 2006·Journal of Neurophysiology·Xiaochuan Guo, Robin A J Lester
Oct 20, 2006·Journal of Neurophysiology·Xiaochuan Guo, Robin A J Lester
Feb 21, 2014·American Journal of Physiology. Cell Physiology·Jin-Young YoonDuk-Su Koh
Mar 1, 2019·Molecular Neuropsychiatry·Heath D SchmidtNii A Addy

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