Time-dependent evolution of tissue markers by MALDI-MS imaging.

Proteomics
Richard J A GoodwinAndrew R Pitt

Abstract

We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room temperature for 30 s to 3 h. Sections were then refrozen, fixed by ethanol treatment and analysed by MALDI-MSI. The intensity of a range of markers were seen to vary across the time course, both increasing and decreasing, with the intensity of some markers changing significantly within 30 s and markers also showed tissue location specific evolution. The markers resulting from this autolysis were compared directly to those that evolved in a comparable 16 h on-tissue trypsin digest, and the markers that evolved in the two studies were seen to be substantially different. These changes offer an important additional level of location-dependent information for mapping changes and seeking disease-dependent biomarkers in the tissue. They also indicate that considerable care is required to allow comparison of biomarkers between MALDI-MSI experiments and also has implications for the standard practice of thaw-mounting multiple tiss...Continue Reading

References

Dec 22, 1999·Analytical Chemistry·P ChaurandR M Caprioli
Nov 22, 2002·Journal of the American Society for Mass Spectrometry·Baogang J XuRoy A Jensen
Feb 14, 2004·Analytical Chemistry·Pierre ChaurandRichard M Caprioli
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Shelley N JacksonJ Albert Schultz
Apr 5, 2005·Toxicologic Pathology·Pierre ChaurandRichard M Caprioli
Aug 9, 2005·Journal of Proteome Research·Michelle L Reyzer, Richard M Caprioli
Feb 2, 2006·Analytical Chemistry·Hans-Rudolf AerniRichard M Caprioli
Feb 7, 2006·Journal of Proteome Research·Karl SköldPer E Andrén
May 18, 2006·Molecular & Cellular Proteomics : MCP·Hélène MeistermannAxel Ducret
Jun 27, 2006·Investigative Ophthalmology & Visual Science·Jun Han, Kevin L Schey
Sep 15, 2006·Analytical Chemistry·Sheerin Khatib-ShahidiRichard M Caprioli
Nov 4, 2006·Journal of Proteome Research·Pierre ChaurandRichard M Caprioli
Mar 21, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shuichi ShimmaMitsutoshi Setou
Nov 10, 2007·Molecular & Cellular Proteomics : MCP·Pierre ChaurandSusan Kasper
Jan 1, 2008·Nature Methods·Malin AnderssonRichard M Caprioli

❮ Previous
Next ❯

Citations

Apr 29, 2010·Chemical Reviews·Kamila Chughtai, Ron M A Heeren
Aug 24, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mohammadreza ShariatgorjiPer E Andrén
Nov 19, 2010·Bioanalysis·Richard J A Goodwin, Andrew R Pitt
May 9, 2014·Bioanalysis·Aurélien Thomas, Pierre Chaurand
Jan 5, 2011·Journal of Cancer Research and Clinical Oncology·S BüngerJ K Habermann
Jun 30, 2012·Journal of Proteomics·Emrys A JonesLiam A McDonnell
Jul 4, 2012·Journal of Proteomics·Kristina Schwamborn
Jun 1, 2010·Journal of Proteomics·Liam A McDonnellAndré M Deelder
Oct 24, 2012·Proteomics. Clinical Applications·Laurens MinervaLutgarde Arckens
Mar 19, 2011·Rapid Communications in Mass Spectrometry : RCM·Richard J A GoodwinC Logan Mackay
May 29, 2012·Rapid Communications in Mass Spectrometry : RCM·Nozomi TakaiHideo Saji
Jul 22, 2014·Journal of Mass Spectrometry : JMS·Nathan Heath PattersonPierre Chaurand
May 21, 2010·Rapid Communications in Mass Spectrometry : RCM·R J GoodwinA R Pitt
Jun 25, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Berin A BoughtonUte Roessner
Mar 15, 2015·British Journal of Pharmacology·D F CobiceR Andrew
Jan 15, 2015·Biochimica Et Biophysica Acta·Jörg HanriederAndrew G Ewing
Nov 7, 2014·Journal of the American Society for Mass Spectrometry·Lukas KrasnyAnna C Crecelius
Mar 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jonathan A DisselhorstBernd J Pichler
Jul 25, 2018·Journal of Neurochemistry·Wojciech MichnoJörg Hanrieder
Dec 19, 2013·Mass Spectrometry·Cecilia ErikssonMitsutoshi Setou
Jan 9, 2020·Journal of the Royal Society, Interface·Axel PetzoldSonia O'Connor
Dec 10, 2016·Archives of Toxicology·Oskar Karlsson, Jörg Hanrieder
Aug 20, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lisa H CazaresO John Semmes
Sep 9, 2020·Journal of the American Society for Mass Spectrometry·Jessica K LukowskiUNKNOWN Kidney Precision Medicine Project
Sep 25, 2021·Mass Spectrometry Reviews·Ashley PhetsanthadLingjun Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.