PMID: 9178731Jun 1, 1997Paper

Time-dependent expression of cytochrome P450 genes in primary cultures of well-differentiated human hepatocytes

The Journal of Laboratory and Clinical Medicine
J GeorgeG C Farrell

Abstract

We sought to establish an in vitro system to study the regulation of highly differentiated hepatocellular functions, and specifically the time-dependent expression of four cytochrome P450 (P450) genes at the messenger RNA (mRNA) and protein levels. When seeded onto matrigel, hepatocytes could be maintained for 8 days in media that were free of serum and hormones (except for insulin). Cells retained a spherical phenotype; they secreted albumin and not alpha-fetoprotein; and the cellular RNA/DNA ratio rose progressively in culture. The isolation procedure and the duration of culture affected expression of specific P450s differently. CYP1A2, CYP2C9, and CYP2E1 mRNAs were not altered by cell isolation, and levels of CYP1A2 and CYP2C9 mRNA were also maintained for 8 days in culture, whereas CYP2E1 mRNA declined to 9% of values in fresh hepatocytes by day 8. CYP3A4 mRNA content was considerably decreased in freshly isolated hepatocytes compared with normal liver, and expression of this gene during the course of culture was more variable than that of the other P450s. Use of Williams' E medium considerably enhanced accumulation of CYP3A4 mRNA, compared with modified Waymouth 752/1 medium, but had a detrimental effect on levels of the o...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 6, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M MauriceP Maurel
Sep 1, 1992·Trends in Pharmacological Sciences·F J Gonzalez
Jan 1, 1992·Critical Reviews in Toxicology·S A Wrighton, J C Stevens
Jan 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M J Gómez-LechónJ V Castell
Jul 31, 1990·European Journal of Biochemistry·F MorelA Guillouzo
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E EliassonM Ingelman-Sundberg
Jun 1, 1985·The Journal of Cell Biology·E G HaymanE Ruoslahti
Mar 1, 1989·Trends in Pharmacological Sciences·F P Guengerich
Jan 28, 1986·Biochemistry·H K KleinmanG R Martin
Aug 1, 1987·Molecular Endocrinology·B J SongF J Gonzalez
Mar 1, 1988·Journal of Cellular Physiology·E G SchuetzP S Guzelian
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L C QuattrochiR H Tukey
Jul 1, 1985·Biochemical Pharmacology·B J BlaauboerA J Paine
Aug 15, 1985·Biochemical Pharmacology·A GuillouzoC Guguen-Guillouzo
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jun 1, 1983·Analytical Biochemistry·D M Durnam, R D Palmiter
Jan 1, 1980·Annals of the New York Academy of Sciences·D M Bissell, P S Guzelian
Nov 28, 1980·Biochemical and Biophysical Research Communications·A J Paine, P Villa
Jan 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J GeorgeG C Farrell
Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E G SchuetzP S Guzelian
Feb 1, 1993·Pharmacology & Therapeutics·U Christians, K F Sewing
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F MorelA Guillouzo

❮ Previous
Next ❯

Citations

May 24, 2001·Archives of Biochemistry and Biophysics·N LiuL S Kaminsky
Jun 7, 2003·Toxicology and Applied Pharmacology·Esther F A BrandonJan H M Schellens
Jul 12, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C EeckhoutteM Dacasto
Sep 15, 1998·Journal of Gastroenterology and Hepatology·C LiddleM Tapner
Jul 27, 2006·Expert Opinion on Drug Metabolism & Toxicology·José V CastellMaría José Gómez-Lechón
Nov 30, 2011·The International Journal of Biochemistry & Cell Biology·Danielle K SeviorJorma T Ahokas
May 13, 2010·Journal of Pharmacological and Toxicological Methods·Marc LübberstedtKatrin Zeilinger
Feb 13, 2001·Toxicology and Applied Pharmacology·D D VakhariaL S Kaminsky
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E EllisC Einarsson
Aug 5, 2003·Journal of Biochemical and Molecular Toxicology·Stefan Wilkening, Augustinus Bader
Mar 3, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Karen MathijsJoost H M van Delft
Aug 6, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·C Rodríguez-AntonaM-J Gómez-Lechón
Dec 20, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N HariparsadP B Desai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Xenobiotica; the Fate of Foreign Compounds in Biological Systems
D M CrossK Wilson
Biochemical and Biophysical Research Communications
M ShimojiT Kamataki
Xenobiotica; the Fate of Foreign Compounds in Biological Systems
M T DonatoM J Gómez-Lechón
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
A E Sirica
© 2021 Meta ULC. All rights reserved