Time-dependent increases in ouabain-sensitive Na+, K+ -ATPase activity in aortas from diabetic rats: The role of prostanoids and protein kinase C

Life Sciences
Luana C GalloLuciana V Rossoni

Abstract

Na(+), K(+)-ATPase activity contributes to the regulation of vascular contractility and it has been suggested that vascular Na(+), K(+)-ATPase activity may be altered during the progression of diabetes; however the mechanisms involved in the altered Na(+), K(+)-ATPase activity changes remain unclear. Thus, the aim of the present study was to evaluate ouabain-sensitive Na(+), K(+)-ATPase activity and the mechanism(s) responsible for any alterations on this activity in aortas from 1- and 4-week streptozotocin-pretreated (50 mg kg(-1), i.v.) rats. Aortic rings were used to evaluate the relaxation induced by KCl (1-10mM) in the presence and absence of ouabain (0.1 mmol/L) as an index of ouabain-sensitive Na(+), K(+)-ATPase activity. Protein expression of COX-2 and p-PKC-betaII in aortas were also investigated. Ouabain-sensitive Na(+), K(+)-ATPase activity was unaltered following 1-week of streptozotocin administration, but was increased in the 4-week diabetic aorta (27%). Endothelium removal or nitric oxide synthase inhibition with l-NAME decreased ouabain-sensitive Na(+), K(+)-ATPase activity only in control aortas. In denuded aortic rings, indomethacin, NS-398, ridogrel or Gö-6976 normalized ouabain-sensitive Na(+), K(+)-ATPase a...Continue Reading

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Citations

Sep 27, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·P S WongR E Roberts
Sep 15, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Nathan R TykockiStephanie W Watts
Apr 20, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yean Chun LohMun Fei Yam
Jun 29, 2016·Pharmacological Reports : PR·Fabiana Vieira SimõesDalton Valentim Vassallo
Oct 1, 2011·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A P DavelL V Rossoni
Jul 18, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhihong TongHaidong Liang
Mar 29, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Hui-Cai GuoYong-Li Wang

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