Time-dependent release of extracellular vesicle subpopulations in tumor CABA I cells

Oncology Reports
Ilaria GiustiVincenza Dolo

Abstract

Investigations into extracellular vesicles (EVs) have significantly increased since their role in physiological and pathological processes has become more clearly understood. Furthermore, it has become increasingly clear that several subpopulations of EVs exist, such as exosomes (EXOs) and microvesicles (MVs). Various methods and techniques used to identify and isolate the specific EVs subpopulations exist. However, these methods should be further elucidated. A deep understanding of the different factors that affect the EVs release may therefore be useful for the standardization of protocols and to establish guidelines for a more adequate analysis and correct inter‑laboratory comparison. In the present study, we investigated whether composition and molecular features of EVs altered over time following a trigger stimulus. Starved CABA I cells were stimulated with FBS and conditioned medium was collected after different time intervals (30 min and 4, 8 and 18 h). The dynamic of EVs release was time-dependent, as shown by the results of scanning electron microscopy. Additionally, the time elapsed from the stimulus affected the size distribution (as highlighted by transmission electron microscopy and NanoSight assay), amount (in ter...Continue Reading

References

Aug 7, 2001·Gastroenterology·G van NielM Heyman
Feb 1, 2006·Molecular and Cellular Neurosciences·J FauréR Sadoul
Dec 17, 2008·Seminars in Thrombosis and Hemostasis·Anoop K EnjetiMichael Seldon
Mar 18, 2009·Clinical Lung Cancer·Guilherme RabinowitsGoetz H Kloecker
Jun 19, 2009·Cell Cycle·Khalid Al-NedawiJanusz Rak
May 7, 2010·Journal of Cell Science·Vandhana Muralidharan-ChariCrislyn D'Souza-Schorey
Jul 6, 2010·Journal of Proteomics·Suresh MathivananRichard J Simpson
Oct 30, 2010·Circulation Research·Sebastian F Mause, Christian Weber
Nov 5, 2010·Seminars in Thrombosis and Hemostasis·Janusz Rak
Nov 1, 2007·Proteomics. Clinical Applications·Eva-Maria Krämer-AlbersJacqueline Trotter
Jan 5, 2011·European Urology·Diederick DuijveszGuido Jenster
Apr 12, 2011·Cancer Research·Joshua L HoodSamuel A Wickline
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Gholamreza TavoosidanaMasood Kamali-Moghaddam
Jun 8, 2011·Traffic·Angélique BobrieClotilde Théry
Oct 26, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Esther N M Nolte-'t HoenMarca H M Wauben
Sep 18, 2012·Advanced Drug Delivery Reviews·Shin-ichiro OhnoMasahiko Kuroda
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Mar 14, 2013·BioMed Research International·Ilaria GiustiVincenza Dolo
Mar 23, 2013·Frontiers in Genetics·Nina Pettersen HessvikAlicia Llorente
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Jan 24, 2014·American Journal of Physiology. Cell Physiology·Giuseppina TurturiciFabiana Geraci
Apr 23, 2014·International Journal of Laboratory Hematology·R van der MeelR M Schiffelers
Jun 3, 2014·Molecular Cancer·Naoomi TominagaTakahiro Ochiya
Oct 4, 2014·Frontiers in Immunology·Zoraida Andreu, María Yáñez-Mó

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