Time dependent response of daunorubicin on cytotoxicity, cell cycle and DNA repair in acute lymphoblastic leukaemia

BMC Cancer
Hussain Mubarak Al-AamriChristopher Bradley

Abstract

Daunorubicin is commonly used in the treatment of acute lymphoblastic leukaemia (ALL). The aim of this study was to explore the kinetics of double strand break (DSB) formation of three ALL cell lines following exposure to daunorubicin and to investigate the effects of daunorubicin on the cell cycle and the protein kinases involved in specific checkpoints following DNA damage and recovery periods. Three ALL cell lines CCRF-CEM and MOLT-4 derived from T lymphocytes and SUP-B15 derived from B lymphocytes were examined following 4 h treatment with daunorubicin chemotherapy and 4, 12 and 24 h recovery periods. Cell viability was measured via MTT (3-(4,5-dimethylthiazol-2-yl)-2-5 diphenyltetrazolium bromide) assay, reactive oxygen species (ROS) production by flow cytometry, double stranded DNA breaks by detecting γH2AX levels while stages of the cell cycle were detected following propidium iodide staining and flow cytometry. Western blotting was used to detect specific proteins while RNA was extracted from all cell lines and converted to cDNA to sequence Ataxia-telangiectasia mutated (ATM). Daunorubicin induced different degrees of toxicity in all cell lines and consistently generated reactive oxygen species. Daunorubicin was more po...Continue Reading

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Citations

Jan 9, 2020·Stem Cell Reviews and Reports·Azin SamimiElahe Khodadi
Sep 18, 2020·Scientific Reports·Vanessa Silva SilveiraCarlos Alberto Scrideli
Sep 23, 2020·Journal of Hematology & Oncology·Andrea Ghelli Luserna di RoràGiorgia Simonetti
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhaohai PanDefang Li
Apr 22, 2021·BMC Cancer·Hussain Mubarak Al-AamriTerri Meehan-Andrews
May 1, 2021·Antioxidants·Saniya ArfinDhruv Kumar
May 29, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Carolina HerreroLorena Alonso-Alconada
Jul 25, 2021·International Journal of Molecular Sciences·Daniel CacicTor Hervig
Sep 7, 2021·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Andy J Moo-MuñozPablo Antonio Moreno-Pérez
Sep 29, 2021·Journal of Experimental & Clinical Cancer Research : CR·Jana PlavaSvetlana Miklikova

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Methods Mentioned

BETA
flow cytometry
protein assay
Reverse
PCR
electrophoresis

Software Mentioned

GraphPad Prism
CLC Genomics Workbench
Syngene

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