Time-Lapse Evaluation of Interactions Between Biodegradable Mg Particles and Cells

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Florencia AlvarezMónica Fernández Lorenzo de Mele

Abstract

Mg-based implants have promising applications as biodegradable materials in medicine for orthopedic, dental, and cardiovascular therapies. During wear and degradation microdebris are released. Time-lapse multidimensional microscopy (MM) is proposed here as a suitable tool to follow, in fixed intervals over 24-h periods, the interaction between cells and particles. Results of MM show interactions of macrophages (J774) with the magnesium particles (MgPa) that led to modifications of cell size and morphology, a decrease in duplication rate, and cell damage. Corrosion products were progressively formed on the surface of the particles and turbulence was generated due to hydrogen development. Changes were more significant after treating MgPa with potassium fluoride. In order to complement MM observations, membrane damage as detected by a lactase dehydrogenase (LDH) assay and mitochondrial activity as detected by a WST-1 assay with macrophages and osteoblasts (MC3T3-E1) were compared. A more significant concentration-dependent effect was detected for macrophages exposed to MgPa than for osteoblasts. Accordingly, complementary data showed that viability and cell cycle seem to be more altered in macrophages. In addition, protein profile...Continue Reading

References

Jan 1, 1991·The Journal of Bone and Joint Surgery. British Volume·S SantavirtaA Eskola
Jan 1, 1988·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S M HorowitzD W Lennox
Sep 1, 1972·Experimental Cell Research·M Takeichi, T S Okada
Jan 1, 1982·The Journal of Bone and Joint Surgery. British Volume·M A FreemanP A Revell
Sep 1, 1994·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·T T Glant, J J Jacobs
Feb 1, 1994·The Journal of Bone and Joint Surgery. American Volume·K J KimH E Rubash
Jan 1, 1994·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H GelbD G Baker
Jun 1, 1993·The Journal of Bone and Joint Surgery. American Volume·S R GoldringT M Wright
Aug 1, 1993·Journal of Biomedical Materials Research·R G DavisR J Williams
Apr 1, 1993·The Journal of Arthroplasty·T P SchmalzriedW H Harris
Dec 1, 1995·Bulletin of Environmental Contamination and Toxicology·D BoeningA M Rossignol
Oct 1, 1995·Calcified Tissue International·S M Horowitz, M A Purdon
Oct 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y TanakaS Eto
Mar 1, 1996·Environmental Health Perspectives·S Hirano, K T Suzuki
Nov 1, 1996·Calcified Tissue International·S M Horowitz, J B Gonzales
Oct 13, 1998·The Journal of Bone and Joint Surgery. British Volume·M LindR L Smith
Aug 26, 2000·Archives of Toxicology·C D AnuradhaS Hirano
Aug 19, 2007·BioTechniques·Lynne Lederman
Oct 16, 2007·BioTechniques·Tony J Collins
Jun 20, 2008·Journal of Biomedical Materials Research. Part a·Wolf-Dieter MuellerMiriam Zeddies
Dec 24, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marco S CaicedoNadim J Hallab
Mar 24, 2010·The Journal of Biological Chemistry·Sarah E BergeronPeter A Rubenstein
Aug 10, 2010·Nature Methods·Michael HeldDaniel W Gerlich
Jun 28, 2011·Computer Methods and Programs in Biomedicine·Johannes HuthHans A Kestler
Jun 30, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ana L Di VirgilioMonica Fernández Lorenzo de Mele
Aug 16, 2011·Colloids and Surfaces. B, Biointerfaces·C A GrilloM A Fernández Lorenzo de Mele
Sep 24, 2011·Biomedical Engineering Online·Arjon TurnipMyung-Yung Jeong
Oct 6, 2011·Journal of Biomedical Materials Research. Part a·María AlcaideMaría Teresa Portolés
Feb 22, 2013·Journal of Biomedical Materials Research. Part a·Rosa María LozanoMaría Lorenza Escudero
Feb 23, 2013·Acta Biomaterialia·L ScheidelerJ Geis-Gerstorfer
Jan 1, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Lin MaoWenjiang Ding
Nov 30, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Olga M PosadaM Helen Grant
Feb 18, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Leonie WoltersJanin Reifenrath

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