Time-lapse monitoring of zona pellucida-free embryos obtained through in vitro fertilization: a retrospective case series

Fertility and Sterility
Daniel BodriTsunekazu Matsumoto

Abstract

To report time-lapse monitoring of human oocytes in which the damaged zona pellucida was removed, producing zona-free (ZF) oocytes that were cultured until the blastocyst stage in time-lapse incubators. Retrospective case series. Private infertility clinic. Infertile patients (n = 32) undergoing minimal ovarian stimulation or natural cycle IVF treatment between October 2012 and June 2014. Intracytoplasmic sperm injection (ICSI) fertilization of ZF oocytes, prolonged embryo culture in time-lapse incubators, elective vitrification, and subsequent single vitrified-thawed blastocyst transfer (SVBT). Rate of fertilization, cleavage and blastocyst development, live-birth rate per SVBT cycle. In spite of advanced maternal age (39 ± 4.2; range, 30-46 years), good fertilization (94%), cleavage (94%), and blastocyst development rates (38%) were reached after fertilization and culturing of ZF oocytes/embryos. All thawed ZF blastocysts survived, and up to this date seven SVBT transfers were performed, yielding three (43%) term live births with healthy newborns. Time-lapse imagery gives a unique insight into the dynamics of embryo development in ZF embryos. Moreover, our case series demonstrate that an oocyte with a damaged zona pellucida t...Continue Reading

References

Oct 21, 1999·Human Reproduction·K TakahashiM Motoyama
May 15, 2010·Reproductive Biomedicine Online·Gábor VajtaBarry D Bavister
Jul 19, 2014·Human Reproduction Update·Kirstine Kirkegaard, Hans Jakob Ingerslev

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Citations

Mar 5, 2016·Fertility and Sterility·Daniel BodriTsunekazu Matsumoto
Feb 10, 2016·Fertility and Sterility·Yunxia Hu, Mark P Trolice
Feb 26, 2021·Journal of Assisted Reproduction and Genetics·Kate WatsonDeirdre Zander-Fox

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