Time-lapse single-cell transcriptomics reveals modulation of histone H3 for dormancy breaking in fission yeast.

Nature Communications
Hayato TsuyuzakiMasamitsu Sato

Abstract

How quiescent cells break dormancy is a key issue in eukaryotic cells including cancer. Fungal spores, for example, remain quiescent for long periods until nourished, although the mechanisms by which dormancy is broken remain enigmatic. Transcriptome analysis could provide a clue, but methods to synchronously germinate large numbers of spores are lacking, and thus it remains a challenge to analyse gene expression upon germination. Hence, we develop methods to assemble transcriptomes from individual, asynchronous spore cells of fission yeast undergoing germination to assess transcriptomic changes over time. The virtual time-lapse analyses highlights one of three copies of histone H3 genes whose transcription fluctuates during the initial stage of germination. Disruption of this temporal fluctuation causes defects in spore germination despite no visible defects in other stages of the life cycle. We conclude that modulation of histone H3 expression is a crucial 'wake-up' trigger at dormancy breaking.

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Mar 31, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sandra B Hake, C David Allis
Apr 25, 2007·Nucleic Acids Research·Yuko Takayama, Kohta Takahashi
May 31, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mizuki ShimanukiMitsuhiro Yanagida
Dec 22, 2009·Bioinformatics·Bo LiColin N Dewey
Nov 1, 2011·Nucleic Acids Research·Valerie WoodStephen G Oliver
Dec 3, 2014·Analytical Biochemistry·Hiroko MatsunagaHideki Kambara
Jan 24, 2017·Nature Methods·Xiaojie QiuCole Trapnell
Aug 22, 2017·Nature Methods·Xiaojie QiuCole Trapnell

❮ Previous
Next ❯

Methods Mentioned

BETA
scRNA-seq
electrophoresis
cDNA library
gene knock-out
PCR

Software Mentioned

R package monocle
SoftWoRx
R package Rtsne
Subio Platform
R package amap
dendextend
dbscan
RSEM
mclust

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.